Effects of Ionic Liquids as Additives on Protein Crystallization.
Effects of Ionic Liquids as Additives on Protein Crystallization.
复制标题
离子液体作为添加剂对蛋白质结晶的影响。
DOI：
10.1007/978-1-0716-3147-8_10
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Pusey,MarcL
中科院分区：
文献类型：
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作者：
Tarver,CrissyL;Pusey,MarcL
蛋白质结晶是一个复杂的过程，其中结晶过程的每个组分和物理参数都可能对结果产生影响。结晶条件通常通过筛选过程来达到，其中目标经受广泛的溶液条件，目的是获得至少一种可以在结构上进行的条件。离子液体（IL）已被发现是用于改善结晶过程的结果的有用添加剂，现有数据表明IL结构具有效果。我们描述了一种快速制备一系列溶液的方法，这些溶液仅在一种组分中变化，在这种情况下，一系列IL用作结晶添加剂。该方法产生筛选网格，其中所测试的结晶条件在Y维度的任何一列中是恒定的，并且它们的IL在X维度的任何一行中是恒定的。这提供了一个系统的方法来确定有效的离子液体从有限的一组有前途的起始结晶条件获得晶体。该方法生成X-Y条件阵列，其中基本沉淀剂条件在一个板维度上保持恒定，添加剂在第二维度上保持恒定，生成12 × 8条件阵列。这种方法也将是有用的调查其他类别的蛋白质结晶添加剂在一个系统的方式。
Protein crystallization is a complex process, where every component and physical parameter of the crystallization process may have an effect on the outcome. Crystallization conditions are typically arrived at by a screening process, where the target is subjected to a broad array of solution conditions with the goal of obtaining at least one condition that can be carried on to a structure. Ionic liquids (IL) have been found to be useful additives for improving the outcomes of the crystallization process, with existing data indicating that the IL structure has an effect. We describe a method for quickly preparing a series of solutions that vary in just one component, in this case a series of ILs that are used as crystallization additives. The method results in a screening grid, where the crystallization conditions being tested are constant in any one column in the Y dimension and they ILs are constant in any one row in the X dimension. This provides a systematic approach to determining effective ILs for obtaining crystals from a limited set of promising starting crystallization conditions. The approach generates an X-Y array of conditions, where the basic precipitant conditions are kept constant in one plate dimension and the additives are kept constant in the second dimension, generating a 12 × 8 array of conditions. This approach would also be useful for surveying other classes of protein crystallization additives in a systematic fashion.