Osteopontin-induced brown adipogenesis from white preadipocytes through a PI3K-AKT dependent signaling
Osteopontin-induced brown adipogenesis from white preadipocytes through a PI3K-AKT dependent signaling
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骨桥蛋白通过 PI3K-AKT 依赖性信号传导从白色前脂肪细胞诱导棕色脂肪生成
DOI：
10.1016/j.bbrc.2015.02.153
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发表时间：
2015
影响因子：
3.1
通讯作者：
Huang Qi-Ren
中科院分区：
文献类型：
--
作者：
Zhong Xiao-Juan;Shen Xiao-Dan;Wen Jian-Bing;Kong Ying;Chu Jia-Jia;Yan Guo-Qiang;Li Teng;Liu Dan;Wu Meng-Qing;Zeng Guo-Hua;He Ming;Huang Qi-Ren
最近的研究表明，骨桥蛋白（OPN）在细胞存活、分化、生物矿化、癌症和心血管重塑中起着关键作用。然而，其在棕色脂肪细胞分化中的作用及其机制尚不清楚。因此，本研究的目的是探讨骨桥蛋白在棕色脂肪形成中的作用及其机制。结果表明，OPN以浓度和时间依赖性方式成功地诱导3 T3-L1白色前脂肪细胞分化为PRDM 16+（PRD 1-BF 1-RIZ 1同源结构域包含16）和UCP-1+（解偶联蛋白-1）棕色脂肪细胞。此外，PI 3 K（磷脂酰肌醇3-激酶）-AKT通路的激活是OPN诱导的棕色脂肪形成所必需的。研究结果表明，OPN在促进棕色脂肪细胞分化方面起着重要作用，可能为肥胖及相关疾病的治疗提供一种潜在的新方法。
Recent studies have shown that OPN (osteopontin) plays critical roles in cell survival, differentiation, bio-mineralization, cancer and cardiovascular remodeling. However, its roles in the differentiation of brown adipocytes and the underlying mechanisms remain unclear. Therefore, the aim of this study was to investigate the roles of OPN in the brown adipogenesis and the underlying mechanisms. It was shown that the OPN successfully induced the differentiation of 3T3-L1 white preadipocytes into the PRDM16+(PRD1-BF1-RIZ1 homologous domain containing 16) and UCP-1+(uncoupling protein-1) brown adipocytes in a concentration and time-dependent manner. Also, activation of PI3K (phosphatidylinositol 3-kinase)-AKT pathway was required for the OPN-induced brown adipogenesis. The findings suggest OPN plays an important role in promoting the differentiation of the brown adipocytes and might provide a potential novel therapeutic approach for the treatment of obesity and related disorders.