Development of PLGA-coated β-TCP scaffolds containing VEGF for bone tissue engineering
Development of PLGA-coated β-TCP scaffolds containing VEGF for bone tissue engineering
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DOI：
10.1016/j.msec.2016.07.011
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发表时间：
2016-12-01
影响因子：
7.9
通讯作者：
Tayebi, Lobat
中科院分区：
文献类型：
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作者：
Khojasteh, Arash;Fahimipour, Farahnaz;Tayebi, Lobat
骨组织工程是指不受自体骨移植或同种异体骨移植或异种骨移植的局限性和短期性的骨缺损修复策略。本研究的目的是制备一种薄层聚乳酸-羟基乙酸共聚物（PLGA）涂层β-磷酸三钙（β-TCP）支架，并持续释放血管内皮生长因子（VEGF）。PLGA涂层显著增加了β-TCP支架的抗压强度。对于体外评价，分离并表征犬间充质干细胞（cMSC）和犬内皮祖细胞（cEPC）。细胞增殖和附着被证实，并且与没有VEGF负载的支架相比，在VEGF释放的支架上的细胞增殖速率显著更高。与其他组相比，在负载有VEGF和MSC的支架中COL 1和RUNX 2的表达显著增加。因此，PLGA涂层的β-TCP支架与VEGF的持续和局部释放显示出良好的结果，在体外骨再生，这种支架有可能在未来用作药物输送装置。(C)2016由Elsevier B. V.出版
Bone tissue engineering is sought to apply strategies for bone defects healing without limitations and short-comings of using either bone autografts or allografts and xenografts. The aim of this study was to fabricate a thin layer poly(lactic-co-glycolic) acid (PLGA) coated beta-tricalcium phosphate (beta-TCP) scaffold with sustained release of vascular endothelial growth factor (VEGF). PLGA coating increased compressive strength of the beta-TCP scaffolds significantly. For in vitro evaluations, canine mesenchymal stem cells (cMSCs) and canine endothelial progenitor cells (cEPCs) were isolated and characterized. Cell proliferation and attachment were demonstrated and the rate of cells proliferation on the VEGF released scaffold was significantly more than compared to the scaffolds with no VEGF loading. A significant increase in expression of COL1 and RUNX2 was indicated in the scaffolds loaded with VEGF and MSCs compared to the other groups. Consequently, PLGA coated beta-TCP scaffold with sustained and localized release of VEGF showed favourable results for bone regeneration in vitro, and this scaffold has the potential to use as a drug delivery device in the future. (C) 2016 Published by Elsevier B.V.