External Volume Expansion Adjusted Adipose Stem Cell by Shifting the Ratio of Fibronectin to Laminin
External Volume Expansion Adjusted Adipose Stem Cell by Shifting the Ratio of Fibronectin to Laminin
复制标题
通过改变纤连蛋白与层粘连蛋白的比例来调节脂肪干细胞的外部体积膨胀
DOI：
10.1089/ten.tea.2019.0095
复制
发表时间：
2018
影响因子：
4.1
通讯作者：
Lu Feng
中科院分区：
文献类型：
--
作者：
Zhang Ziang;Cai Junrong;Li Ye;He Yunfan;Dong Ziqing;Dai Jingxing;Lu Feng
体外容积扩张术是一种有效的脂肪组织再生方法。然而，目前还不清楚EVE如何诱导脂肪组织再生。在这项研究中，我们开发了EVE设备，以产生扩大预制脂肪组织（EPAT）在大鼠和研究细胞增殖，脂肪形成，细胞外基质（ECM）蛋白的表达在12周的吸力。此外，使用EPAT产生的脱细胞脂肪组织（DAT）来评估ECM蛋白在细胞增殖和分化中的作用。EVE抽吸后基质沉积显著增加，其中纤维连接蛋白和层粘连蛋白显示出最显著的变化。纤连蛋白表达在第1-4周达到峰值，此时EPAT中的Ki 67细胞达到峰值。层粘连蛋白的表达在第8-12周达到高峰，过氧化物酶体增殖物激活受体-γ的表达也达到高峰。在体外，脂肪源性干细胞（ASC）在第1周DAT时显示出较高的增殖率，此时纤维连接蛋白表达最高，而ASC在第12周DAT时脂肪生成显著较高，此时层粘连蛋白表达丰富。这些结果表明，EVE装置增强了ECM沉积，这与细胞增殖和分化密切相关。Impact StatementLarge soft tissue defects caused by cancer，trauma，or remediation remains a major challenge for reconstructive surgery.体外容积扩张术（EVE）成功地诱导了脂肪组织再生，在软组织缺损的矫正中显示出巨大的治疗潜力。本研究表明，EVE增强细胞外基质（ECM）蛋白的分泌，并通过将基质合成从纤连蛋白转移到层粘连蛋白来调节ASC的增殖和分化。这些发现揭示了ECM调节和ASC行为之间的关系，表明EVE可以通过调节基质合成来诱导脂肪再生。
External volume expansion (EVE) is an effective method of adipose tissue regeneration. However, it remains unclear how EVE induces adipose tissue regeneration. In this study, we developed EVE devices to generate expanded prefabricated adipose tissue (EPAT) in rats and investigated cell proliferation, adipogenesis, and the expression of extracellular matrix (ECM) proteins during the 12 weeks suction. In addition, EPAT-generated decellularized adipose tissue (DAT) was used to assess the role of ECM proteins in cell proliferation and differentiation. Matrix deposition was significantly increased after EVE suction, with fibronectin and laminin showing the most dramatic changes. Fibronectin expression peaked during weeks 1–4, when Ki67 cells in EPAT peaked. Laminin expression peaked during weeks 8–12, when peroxisome proliferator-activated receptor-γ expression also peaked.In vitro, adipose-derived stem cells (ASCs) displayed a higher proliferation rate in week 1 DAT, when fibronectin expression was highest, whereas ASC adipogenesis was significantly higher on week 12 DAT, when laminin expression was abundant. These results showed that EVE device enhanced ECM deposition, which is closely related to cell proliferation and differentiation.Impact StatementLarge soft tissue defects caused by cancer, trauma, or deformity remain a major challenge for reconstructive surgery. External volume expansion (EVE) successfully induces adipose tissue regeneration and shows great therapeutic potential in correction for soft tissue defect. This study showed that EVE enhanced the secretion of extracellular matrix (ECM) proteins and regulated ASC proliferation and differentiation through shifting matrix synthesis from fibronectin to laminin. These findings revealed the relation between ECM modulation and ASC behavior, indicating that EVE can induce adipose regeneration by regulating matrix synthesis.