RADIATION-INDUCED CLASTOGENIC FACTORS - ANTICLASTOGENIC EFFECT OF GINKGO-BILOBA EXTRACT
RADIATION-INDUCED CLASTOGENIC FACTORS - ANTICLASTOGENIC EFFECT OF GINKGO-BILOBA EXTRACT
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DOI：
10.1016/0891-5849(94)00220-e
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发表时间：
1995-06-01
影响因子：
7.4
通讯作者：
ASRIAN, K
中科院分区：
文献类型：
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作者：
EMERIT, I;ARUTYUNYAN, R;ASRIAN, K
致染色体断裂因子（CF）最早是在意外或治疗性照射的人的血液中发现的。我们实验室的工作表明，它们也发生在其他情况下，其特征是氧化应激，CF诱导的染色体损伤定期防止超氧化物歧化酶（SOD）。最近，我们在切尔诺贝利反应堆事故的救援人员中发现了很高比例的CF。这些清理者代表了高风险人群，可能受益于抗氧化剂的癌症化学预防。SOD必须注射，不适合长期预防性治疗。因此，在本研究中，我们评估了银杏叶提取物EGb 761的抗胚泡作用，这是已知的超氧化物清除性能。EGb 761在CF处理的健康供体血培养物中进行了检测。在建立了最佳的保护EGb浓度，使用CFs从健康志愿者的全血照射产生的，提取物进行了测试，暴露于CFs从血浆照射的人作为清算人的文化。最终浓度为100 μ g/ml时，可证实抗胚泡生成作用。在12个连续实验中，CF诱导平均18.00 +/-4.41畸变/100个细胞。在接受100 μ g/ml EGb 761的平行培养物中，该值降低至7.33 +/- 3.08（p < .001）。SOD是anticlastogenic在相同的系统中，在30细胞色素C单位/ml（约10 μ g/ml）的浓度。在一个小系列的清理者中获得的初步结果显示，在用EGb 761（3 x 40 mg/d）治疗2个月后，血浆中的CF消退或完全消失。
Clastogenic factors (CFs) were first described in the blood of persons irradiated accidentally or for therapeutic reasons. Work of our laboratory has shown that they occur also under other circumstances, which are characterized by oxidative stress, and that CF-induced chromosome damage is regularly prevented by superoxide dismutase (SOD). Recently we found CFs in a high percentage of salvage personnel of the Chernobyl reactor accident. These liquidators represent a high-risk population and might benefit from cancer chemoprevention by antioxidants. SOD would have to be injected and is not appropriate for longterm prophylactic treatment. In the present study, we therefore evaluated the anticlastogenic effect of the Ginkgo biloba extract EGb 761, which is known for its superoxide scavenging properties. EGb 761 was tested on CF-treated blood cultures of healthy donors. After establishing the optimal protective EGb concentration, using CFs produced by irradiation of whole blood from healthy volunteers, the extract was tested on cultures exposed to CFs from plasma of persons irradiated as liquidators. The anticlastogenic effect could be confirmed for a final concentration of 100 mu g/ml. In 12 consecutive experiments, CFs induced an average of 18.00 +/- 4.41 aberrations/100 cells. This was reduced to 7.33 +/- 3.08 in the parallel cultures receiving 100 mu g/ml EGb 761 (p < .001). SOD was anticlastogenic in the same system at concentrations of 30 cytochrome C units/ml (approximately 10 mu g/ml). Preliminary results obtained in a small series of liquidators showed regression or complete disappearance of CFs in the plasma after 2 months of treatment with EGb 761 (3 x 40 mg/d).