Gene expression of tumor necrosis factor α and TNF-receptors, p55 and p75, in nonalcoholic steatohepatitis patients
Gene expression of tumor necrosis factor α and TNF-receptors, p55 and p75, in nonalcoholic steatohepatitis patients
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DOI：
10.1053/jhep.2001.29628
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发表时间：
2001-12-01
期刊：
影响因子：
13.5
通讯作者：
Pons-Romero, F
中科院分区：
文献类型：
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作者：
Crespo, J;Cayón, A;Pons-Romero, F
本研究的主要目的是分析肿瘤坏死因子- α (tnf - α)系统在非酒精性脂肪性肝炎（NASH）发展中的致病作用。对52例肥胖患者进行了研究。我们通过定量逆转录酶聚合酶链反应（RT-PCR）研究了：(1)肝脏和脂肪组织中tnf - α及其p55和p75受体mRNA的表达；(2)tnf - α、p55和p75与NASH严重程度的关系。无NASH的肥胖患者为对照组。NASH患者肝组织（0.65 +/- 0.54）和外周脂肪（0.43 +/- 0.45）中tnf - α mRNA表达显著升高；对照组中，mRNA表达为0.28 +/- 0.32，P
The main objective of this study was to analyze the pathogenic role of the tumor necrosis factor alpha (TNF-alpha) system in the development of nonalcoholic steatohepatitis (NASH). Fifty-two obese patients were studied. We investigated: (1) the expression of mRNA of TNF-alpha and their p55 and p75-receptors by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR) in hepatic and adipose tissues, and (2) the relationship between TNF-alpha, p55, and p75 and the severity of NASH. Obese patients without NASH were the control group. A remarkable increase in the expression of mRNA of TNF-alpha was found in patients with NASH in hepatic tissue (0.65 +/- 0.54) and in peripheral fat (0.43 +/- 0.45): in the control samples, the mRNA expression was 0.28 +/- 0.32, P