Modeling and biological implication of time-dose-mortality data for the entomophthoralean fungus, Zoophthora anhuiensis, on the green peach aphid Myzus persicae
Modeling and biological implication of time-dose-mortality data for the entomophthoralean fungus, Zoophthora anhuiensis, on the green peach aphid Myzus persicae
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DOI：
10.1006/jipa.1998.4794
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发表时间：
1998-11-01
影响因子：
3.4
通讯作者：
Xu, Q
中科院分区：
文献类型：
--
作者：
Feng, MG;Liu, CL;Xu, Q
安徽虫瘟霉（Zoophthoraanhuiensis）在秋末冬初常引起危害长江中下游十字花科作物的绿色桃蚜种群流行。在这项研究中，进行了生物测定暴露在离体叶片上的蚜虫不同剂量的Z。离体培养释放出的分生孢子。使用10个剂量（1.5-198分生孢子/mm（2）），每个剂量包括64-120个蚜虫。将蚜虫保持在18 ℃下，12：12（L：D）的温度下，每天观察死亡率。基于Hosmer-Lemeshow检验，所得时间-剂量-死亡率数据与条件死亡率概率模型拟合良好（（C）over cap = 9.52，df = 8，P = 0.70），从而得到累积死亡率概率模型。后一模型的参数用于估计真菌物种对害虫的致死剂量（LD 50）和时间（LT 50）。在暴露后第5、6和7天，LD_（50）值分别为87、44和34分生孢子/mm（2）。估计的LT 50值从37分生孢子/mm（2）的6.7天降低到198分生孢子/mm（2）的4.5天。结果表明，Z.安徽虫瘟霉是一种很有前途的微生物防治病原菌。该模型推荐用于真菌-昆虫协会的时间-剂量-死亡率数据的分析。的生物学意义的参数和建模技术的优势比传统的方法的概率分析进行了讨论。(C)北京：科学出版社.
The entomophthoralean fungus Zoophthora anhuiensis frequently causes epizootics in populations of the green peach aphid, Myzus persicae, infesting cruciferous crops in the middle and lower regions of the Changjiang River, China, during late autumn and early winter. In this study, a bioassay was conducted by exposing aphids on detached leaves to varying doses of Z. anhuiensis conidia discharged from in vitro cultures. Ten doses (1.5-198 conidia/mm(2)) were used, with each dose including 64-120 aphids. The aphids were maintained at 18 degrees C under a photophase of 12:12 (L:D) and observed daily for mortality. The resulting time-dose-mortality data fitted well to a conditional mortality probability model based on the Hosmer-Lemeshow test ((C) over cap = 9.52, df = 8, P = 0.70), resulting in a cumulative mortality probability model. The parameters from the latter model were used to estimate the lethal dose (LD50) and time (LT50) for the fungal species against the pest. The LD50 values were 87, 44, and 34 conidia/mm(2) on days 5, 6, and 7 after exposure, respectively. The estimates of the LT50 values decreased from 6.7 days at 37 conidia/mm(2) to 4.5 days at 198 conidia/mm(2). The results indicate that Z. anhuiensis could be a promising aphid pathogen for microbial control competitive with other Zoophthora species. The model is recommended for use in the analysis of time-dose-mortality data for fungus-insect associations. The biological implications of the parameters and the advantages of the modeling technique over the conventional method of probit analysis are discussed. (C) 1998 Academic Press.