MODULATION OF RAT AND HUMAN LYMPHOCYTE FUNCTION BY N-6 AND N-3 POLYUNSATURATED FATTY-ACIDS AND ACETYLSALICYLIC-ACID
MODULATION OF RAT AND HUMAN LYMPHOCYTE FUNCTION BY N-6 AND N-3 POLYUNSATURATED FATTY-ACIDS AND ACETYLSALICYLIC-ACID
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DOI：
10.1159/000177763
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发表时间：
1993-05-01
影响因子：
3.9
通讯作者：
PASCAUD, M
中科院分区：
文献类型：
--
作者：
BROUARD, C;PASCAUD, M
研究了体外添加10 ~ 100个muM n-6和n-3多不饱和脂肪酸对体外培养的受刺激T淋巴细胞增殖的影响。对于植物血凝素诱导的人血液淋巴细胞和豆豆素a诱导的大鼠脾淋巴细胞，22:4 n-6和22:6 n-3的抑制作用最大，20:5 n-3的抑制作用较小。花生四烯酸20:4 n-6对大鼠淋巴细胞增殖无抑制作用。乙酰水杨酸对人和大鼠淋巴细胞增殖均有刺激作用。研究了适度降低亚麻油和鱼油中最大二十碳五烯酸（MaxEPA）对大鼠淋巴细胞增殖和NK细胞活性的影响。饮食变化不影响体外有丝分裂原诱导的淋巴细胞增殖，但n-3富集饮食显著降低了未刺激淋巴细胞的增殖（4倍）。鱼油显著提高大鼠脾淋巴细胞NK细胞活性，而亚麻籽油不显著提高。富n-3饲粮，特别是鱼油饲粮降低了体外添加乙酰水杨酸（阿司匹林）对淋巴细胞增殖的刺激作用。
The effects of in vitro additions of between 10 and 100 muM n-6 and n-3 polyunsaturated fatty acids were examined on the proliferation of stimulated T lymphocytes in culture. For both phytohemagglutinin-induced human blood lymphocytes and concanavalin-A-induced rat splenic lymphocytes, the largest inhibitory effects were obtained with 22:4 n-6 and 22:6 n-3, and to a lesser extent with 20:5 n-3. Arachidonic acid 20:4 n-6, the main eicosanoid presursor, was not inhibitory, it even stimulated rat lymphocyte proliferation. Acetylsalicylic acid stimulated both human and rat lymphocyte proliferation. The effects of moderate decreases in the dietary n-6/n-3 ratio by either linseed oil or fish oil maximum eicosapentaenoic acid (MaxEPA) were determined on rat lymphocyte proliferation and natural killer (NK) cell activity in vitro. Dietary changes did not affect mitogen-induced lymphocyte proliferation in vitro, but proliferation of unstimulated lymphocytes was significantly lowered (4-fold) with the n-3-enriched diets. Dietary fish oil but not linseed oil significantly increased the NK cell activity of rat splenic lymphocytes. The n-3-enriched diets, especially the fish oil diets, reduced the stimulatory effect of in vitro added acetylsalicylic acid (aspirin) on lymphocyte proliferation.