The morphological transition of Helicobacter pylori cells from spiral to coccoid is preceded by a substantial modification of the cell wall
The morphological transition of Helicobacter pylori cells from spiral to coccoid is preceded by a substantial modification of the cell wall
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DOI：
10.1128/jb.181.12.3710-3715.1999
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发表时间：
1999-06-01
影响因子：
3.2
通讯作者：
García-del Portillo, F
中科院分区：
文献类型：
--
作者：
Costa, K;Bacher, G;García-del Portillo, F
采用高效液相色谱和质谱技术研究了幽门螺杆菌的肽聚糖（胞壁素）。来自H. pylori对应于Al γ化学型，但胞肽洗脱模式与大肠杆菌的胞肽洗脱模式实质上不同，前者产生高比例的具有五肽侧链（约60 mol%）、具有Gly残基作为C-末端氨基酸（5 - 10 mol%）和具有（1->6）脱水-N-乙酰胞壁酸（13 - 18 mol%）的胞肽。H.幽门螺杆菌胞壁素也缺乏结合胞壁素的脂蛋白、三聚胞壁肽和（L-D）交联胞壁肽。停止生长和过渡到球形形状引发的N-乙酰葡糖胺基-N-乙酰胞壁酰-L-Ala-D-Glu（约20摩尔%）的增加，显然是在牺牲单体muropeptides与三-和四肽侧链。在静息细胞中，含有（1->6）脱水胞壁酸和甘氨酸的胞肽也更丰富。
The peptidoglycan (murein) of Helicobacter pylori has been investigated by high-performance liquid chromatography and mass spectrometric techniques. Murein from H. pylori corresponded to the Al gamma chemotype, but the muropeptide elution patterns were substantially different from the one for Escherichia coli in that the former produced high proportions of muropeptides with a pentapeptide side chain (about 60 mol%), with Gly residues as the C-terminal amino acid (5 to 10 mol%), and with (1-->6)anhydro-N-acetylmuramic acid (13 to 18 mol%). H. pylori murein also lacks murein-bound lipoprotein, trimeric muropeptides, and (L-D) cross-linked muropeptides. Cessation of growth and transition to coccoid shape triggered an increase in N-acetylglucosaminyl-N-acetylmuramyl-L-Ala-D-Glu (approximately 20 mol%), apparently at the expense of monomeric muropeptides with tri- and tetrapeptide side chains. Muropeptides with (1-->6) anhydro-muramic acid and with Gly were also more abundant in resting cells.