Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes
Implication of mycelium-associated laccase from Irpex lacteus in the decolorization of synthetic dyes
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DOI：
10.1016/j.biortech.2007.01.019
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发表时间：
2008-02-01
影响因子：
11.4
通讯作者：
Kuees, U.
中科院分区：
文献类型：
--
作者：
Svobodova, K.;Majcherczyk, A.;Kuees, U.
白腐真菌Irpex lacteus能够脱色活性橙16和Remazol Brilliant Blue r等合成染料。在这里，我们证明了这种染料脱色主要与真菌菌丝体相关的漆酶样酶活性有关。在其结合形式下，检测到的酶对ABTS、DMP和愈创木酚的最佳氧化pH为3.0，对丁香醛的最佳氧化pH为7.0。以ABTS为底物酶活性最高。50mm NaN3完全抑制酶活性。根据染料的化学结构，氧化还原介质对真菌菌丝体对染料的脱色有积极的影响。从真菌菌丝体中分离的酶能够在体外对合成染料进行脱色。(c) 2007 Elsevier Ltd.版权所有。
The white rot fungus Irpex lacteus is able to decolorize such synthetic dyes as Reactive Orange 16 and Remazol Brilliant Blue R. Here, we demonstrate that this type of dye decolorization is mainly related to a laccase-like enzyme activity associated with fungal mycelium. In its bound form, the enzyme detected showed a pH optimum of 3.0 for the oxidation of ABTS, DMP and guaiacol, and a pH of 7.0 for syringaldazine. The highest enzymatic activity was obtained with ABTS as substrate. Enzyme activity was fully inhibited with 50 mM NaN3. Depending on the chemical structure of dyes, redox mediators had a positive effect on the dye decolorization by fungal mycelium. Enzyme isolated from fungal mycelium was able to decolorize synthetic dyes in vitro. (c) 2007 Elsevier Ltd. All rights reserved.