GAMETOCYTOGENESIS OF PLASMODIUM-FALCIPARUM INVITRO - AN ELECTRON-MICROSCOPIC STUDY
GAMETOCYTOGENESIS OF PLASMODIUM-FALCIPARUM INVITRO - AN ELECTRON-MICROSCOPIC STUDY
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DOI：
10.1017/s003118200005160x
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发表时间：
1982-01-01
期刊：
影响因子：
2.4
通讯作者：
SINDEN, RE
中科院分区：
文献类型：
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作者：
SINDEN, RE
从自然感染冈比亚病人的血液中体外培养恶性疟原虫，并用光学显微镜和电子显微镜观察培养的性寄生虫的发育。幼体(II期和III期)雌配子体经历单一的隐裂核分裂。这种分裂紧跟在Sinden et Smolley(1979)在两性配子体的I和II阶段表现出来的S阶段之后。雄配子体在红细胞内的成熟期不进行有丝分裂，保持多倍体。因此，雄配子体和雌配子体的细胞周期明显不同。发育中配子体形状特征变化的超微结构基础被证明是由于膜下膜和微管细胞骨架的组装和随后的丢失。I-III期配子细胞合成大量核糖体和内质网。这与这些幼年寄生虫中RNA和蛋白质的活跃合成有关(Sinden et SMalley，1979)。成熟寄生虫的大分子合成显著减少的同时，只有雄配子体的细胞质核糖体密度降低。在雌性，这种活动的减少与核仁的出现有关。这些变化表明，不同的机制正在被用来控制两性配子体的RNA合成。
P. falciparum was grown in vitro in blood taken from naturally infected Gambian patients, and the development of the cultured sexual parasites was studied by light microscopy and EM. The young (Stage II and III) female gametocytes undergo a single cryptomitotic nuclear division. This division immediately follows the S phase which Sinden et Smalley (1979) demonstrated in the Stage I and II gametocytes of both sexes. The male gametocytes do not undergo mitosis during their maturation period in the erythrocyte and remain polyploid. Hence the cell cycles of the male and female gametocytes differ significantly. The ultrastructural basis of the characteristic changes in shape of the developing gametocyte are shown to be due to the assembly and subsequent loss of components of the subpellicular membranous and microtubular cytoskeleton. Stage I-III gametocytes synthesize numerous ribosomes and endoplasmic reticulum. This correlates with the active synthesis of RNA and protein in these young parasites (Sinden et Smalley, 1979). The marked reduction in macromolecular synthesis in the mature parasites is paralleled by a reduction in cytoplasmic ribosome density in the male gametocyte only. In the female this reduction in activity is correlated with the appearance of a nucleolus. These changes suggest that different mechanisms are being used to control RNA synthesis in the 2 sexes of gametocyte.