Mapping and validation of a dominant salt tolerance gene in the cultivated soybean (Glycine max) variety Tiefeng 8
Mapping and validation of a dominant salt tolerance gene in the cultivated soybean (Glycine max) variety Tiefeng 8
复制标题
栽培大豆（Glycine max）品种铁丰8号显性耐盐基因的定位和验证
DOI：
10.1016/j.cj.2014.09.001
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发表时间：
2014-12-01
期刊：
影响因子：
6.6
通讯作者：
Qiu, Li-juan
中科院分区：
文献类型：
--
作者：
Guan, Rongxia;Chen, Jiangang;Qiu, Li-juan
盐是一种非生物胁迫因子，强烈影响大豆的生长和产量。单个显性基因已被证明赋予大豆品种铁丰 8 号耐盐性。本研究的目的是对 F-2:3 群体和源自两种栽培大豆铁丰 8 号（耐性）和 85-140（敏感）杂交的重组自交系 (RIL) 群体中的耐盐性基因进行遗传图谱分析。 F-2:3家族和RIL用200 mmol L-1 NaCl处理以评价耐盐性。 F-2:3群体显示1（42个耐受）：2（132个分离）：1（65个敏感）分离，表明铁丰8号具有单一的耐盐显性基因。在定位区域内开发了来自先前鉴定的随机扩增多态性DNA（RAPD）标记的序列特征扩增区（SCAR）标记和四个插入/缺失多态性（InDel）标记。使用这些标记和 SSR 标记，将耐盐基因定位在 209 kb 内，两侧是 3 号染色体上的 SCAR 标记 QS08064 和 SSR 标记 Barcsoyssr_3_1301。使用与耐盐基因和 SCAR 标记 QS08064 共分离的三个标记对 35 个耐盐大豆品种和 23 个敏感大豆品种进行基因分型。这些标记显示选择效率为 76.2% 至 94.2%。结果表明，这些标记将有助于标记辅助育种和促进大豆耐盐基因的图位克隆。 (C) 2014 中国作物学会、中国农业科学院作物科学研究所。由 Elsevier B.V. 制作和托管。保留所有权利。
Salt is an abiotic stress factor that strongly affects soybean growth and production. A single dominant gene has been shown to confer salt tolerance in the soybean cultivar Tiefeng 8. The objective of the present study was to genetically map the salt-tolerance gene in an F-2:3 population and a recombinant inbred line (RIL) population derived from a cross between two cultivated soybeans, Tiefeng 8 (tolerant) and 85-140 (sensitive). The F-2:3 families and RILs were treated with 200 mmol L-1 NaCl to evaluate salt tolerance. The F-2:3 population showed 1 (42 tolerant): 2 (132 segregating): 1 (65 sensitive) segregation, indicating a single dominant gene for salt tolerance in Tiefeng 8. A sequence-characterized amplified region (SCAR) marker from a previously identified random amplified polymorphic DNA (RAPD) marker and four insertion/deletion polymorphism (InDel) markers were developed within the mapping region. Using these markers along with SSR markers, the salt-tolerance gene was mapped within 209 kb flanked by SCAR marker QS08064 and SSR marker Barcsoyssr_ 3_ 1301 on chromosome 3. Three markers that cosegregated with the salt tolerance gene and SCAR marker QS08064 were used to genotype 35 tolerant and 23 sensitive soybean accessions. These markers showed selection efficiencies of 76.2% to 94.2%. The results indicate that these markers will be useful for marker-assisted breeding and facilitating map-based cloning of the salt tolerance gene in soybean. (C) 2014 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. All rights reserved.