Distinct immunomodulatory properties of Lactobacillus paracasei strains
Distinct immunomodulatory properties of Lactobacillus paracasei strains
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DOI：
10.1111/j.1365-2672.2011.05147.x
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发表时间：
2011-12-01
影响因子：
4
通讯作者：
Sisto, A.
中科院分区：
文献类型：
--
作者：
D'Arienzo, R.;Bozzella, G.;Sisto, A.
目的：探讨副乳杆菌菌株的免疫调节作用。方法与结果：利用荧光扩增片段长度多态性技术对菌株进行遗传分化，获得了各菌株特有的分子标记。为了确定免疫调节特性，我们评估了菌株对树突状细胞成熟、葡聚糖摄取、诱导同种异体T细胞增殖的能力和细胞因子分泌的影响。结果表明，所有菌株都能刺激树突状细胞(DC)的表型成熟，但它们对DC的作用不同于其他所测试的特性，特别是对细胞因子分泌的影响不同。结论：本研究结果揭示了不同菌株的免疫调节特性。帕拉卡塞。IMPC 4.1菌株显示出一种有趣的抗炎能力。益生菌菌株IMPC2.1和LMGP-17806诱导细胞因子分泌的能力相似和中等，而菌株LMG23554的诱导细胞因子分泌能力很低。研究的意义和影响：我们的结果证实，每一株细菌物种似乎都以一种特殊的方式影响免疫系统。评估不同类型和/或水平的细胞因子的分泌是由每个菌株诱导的，这可能有助于确定其促炎或抗炎特性及其更合适的临床应用。
Aims: This study was performed to ascertain the immunomodulatory effect of Lactobacillus paracasei strains. These strains were also genetically characterized.Methods and Results: The strains were genetically differentiated by using the fluorescent-amplified fragment length polymorphism technique, which led to the identification of several molecular markers unique to each strain. To determine the immunomodulatory properties, we evaluated the effect of strains on dendritic cell maturation, dextran uptake, ability to induce proliferation of allogenic T cells and cytokine secretion. The results indicated that all the strains stimulated phenotypic maturation of dendritic cells (DCs), but they acted differently on DCs in relation to the other tested properties; notably, a different effect on cytokine secretion was detected.Conclusions: The results of this study revealed different immunomodulatory properties of strains of the species Lact. paracasei. Strain IMPC 4.1 showed an interesting anti-inflammatory ability. Probiotic strains IMPC 2.1 and LMG P-17806 were characterized by a similar and intermediate ability to induce cytokine secretion in contrast to the very low ability of strain LMG 23554.Significance and Impact of Study: Our results confirm that each single strain of a bacterial species appears to influence the immune system in a peculiar manner. The evaluation of the different types and/or levels of cytokines whose secretion is induced by each strain could be relevant to define its pro- or anti-inflammatory properties and its more appropriate clinical use.