HISTOCHEMICAL DEMONSTRATION OF REDUCED PYRIDINE NUCLEOTIDE DEPENDENT POLYCYCLIC HYDROCARBON METABOLIZING SYSTEMS
HISTOCHEMICAL DEMONSTRATION OF REDUCED PYRIDINE NUCLEOTIDE DEPENDENT POLYCYCLIC HYDROCARBON METABOLIZING SYSTEMS
复制标题
还原吡啶核苷酸依赖的多环烃代谢系统的组织化学展示
DOI：
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发表时间：
1962
期刊：
影响因子：
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通讯作者：
J. Leong
中科院分区：
文献类型：
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作者：
L. Wattenberg;J. Leong
1. 已经开发了组织化学程序来证明还原吡啶核苷酸依赖系统代谢3,4 -苯并芘，苝，9,10-二甲基-1,2-苯并蒽和1,2-苯并蒽。2. 在这些过程中，采用了原位指示技术，通过改变涂覆在组织切片上的底物的荧光来显示酶活性的位点。3. 在大鼠、小鼠、仓鼠、猴子和人的肝细胞中发现了阳性反应。在所有种类中，肝小叶中心部分的细胞比周围部分的细胞更活跃。4. 对大鼠组织的调查显示，在肾脏近曲小管的远端部分、肾上腺的束状带和网状带、睾丸的间质细胞、甲状腺的滤泡上皮和肺的肺泡壁也有阳性反应。5. 注射甲基胆蒽的大鼠肝脏、肾脏、甲状腺、睾丸和肺组织的活性明显高于对照动物；在肾上腺中，没有发现这种增加。
1. Histochemical procedures have been developed for the demonstration of reduced pyridine nucleotide dependent systems metabolizing 3, 4-benzypyrene, perylene, 9,10-dimethyl-1,2-benzanthracene and 1, 2-benzanthracene. 2. For these procedures, an in situ indicator technique has been employed in which alterations of the fluorescence of the substrates coated on tissue sections is used to demonstrate the sites of enzyme activity. 3. Positive reactions have been found in the hepatic cells of rat, mouse, hamster, monkey, and man. The cells of the central portion of the hepatic lobule are more active than those of the periphery in all species. 4. A survey of rat tissues has shown positive reactions also in the distal portion of the proximal convoluted tubules of the kidney, the zona fasciculata and zona reticularis of the adrenal, the interstitial cells of the testis, the follicular epithelium of the thyroid and the alveolar walls of the lung. 5. The activity is considerably higher in sections of liver, kidney, thyroid, testis, and lung from rats injected with methylcholanthrene than in control animals; in the adrenal, no such increase is found.