DEVELOPMENTAL GENETICS OF HEXOKINASE ISOENZYMES IN DROSOPHILA-MELANOGASTER
DEVELOPMENTAL GENETICS OF HEXOKINASE ISOENZYMES IN DROSOPHILA-MELANOGASTER
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DOI：
10.1016/0022-1910(72)90231-4
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发表时间：
1972-01-01
影响因子：
2.2
通讯作者：
FOX, DJ
中科院分区：
文献类型：
--
作者：
MADHAVAN, K;URSPRUNG, H;FOX, DJ
果蝇（Drosophila melanogaster）单蝇的琼脂凝胶电泳显示了三种形式的己糖激酶，称为Hex-1、Hex-2和Hex-3。Hex-3阴极的另一条轻度染色条带仅存在于雄性果蝇浸提液中。70实验室股票的调查产生了三个电泳变异的六角-3：一个'快'，一个'中间'，和一个'慢'的变种，但没有变异的六角-1或六角-2。利用第二染色体作图stockal dp B pr c px sp，确定Hex-3图谱位置为73±单位。新鲜产下的卵的提取物以及不同的幼虫、蛹和成虫阶段的酶谱表明，Hex-2在发育的所有阶段都是活性的。Hex-3存在于发育的所有阶段，除了新鲜产下的蛋。在总匀浆中，Hex-1和雄性特异性条带仅限于成体发育的晚期。个别幼虫和成人组织的酶谱。
Agar gel electrophoresis of single flies ofDrosophila melanogasterreveals three forms of hexokinase, called Hex-1, Hex-2, and Hex-3. A further lightly staining band cathodal to Hex-3 is present in extracts of male flies only. A survey of 70 laboratory stocks yielded three electrophoretic variants of Hex-3: a ‘fast’, an ‘intermediate’, and a ‘slow’ variant, but no variants of Hex-1 or Hex-2. Using the second chromosome mapping stockal dp b pr c px sp, the Hex-3 map position was determined to be 73± units. Zymograms of extracts of freshly laid eggs, and different larval, pupal and adult stages show that Hex-2 is active in all stages of development. Hex-3 is present in all stages of development except in freshly laid eggs. In total homogenates, Hex-1 and the male-specific band are restricted to late stages of adult development. Zymograms of individual larval and adult tissues are presented.