A better cell line for making hybridomas secreting specific antibodies
A better cell line for making hybridomas secreting specific antibodies
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用于制造分泌特异性抗体的杂交瘤的更好细胞系
作者：
M. Shulman;C. Wilde;G. Köhler
在组织培养物中生长的骨髓瘤细胞与来自免疫小鼠的脾细胞的融合提供了用于获得细胞系（杂交瘤）的通用方法，所述细胞系（杂交瘤）产生所需特异性的抗体1 -3。源自这些骨髓瘤的杂交体产生骨髓瘤亲本的免疫球蛋白（IG）重链和轻链以及脾细胞亲本的抗原特异性重链和轻链。在两条重链和两条轻链随机结合的条件下，杂交瘤将产生10个不同的IG分子，并且特异性抗体将仅包含总Ig的1/16 4，5。为了获得仅产生特异性抗体的杂交瘤，需要本身不产生IG但仍能与脾细胞融合以获得仅分泌特异性抗体的杂交瘤的肿瘤细胞融合配偶体。我们在这里报告这样的细胞系，Sp2/0-Ag 14的鉴定。
FUSION of myeloma cells which grow in tissue culture with spleen cells from an immunised mouse provides a general method for obtaining cell lines (hybridomas) which make antibody of the desired specificity1–3. Hybrids derived from these myelomas make the immunoglobulin (Ig) heavy and light chains of the myeloma parent as well as the antigen-specific heavy and light chains of the spleen cell parent. In conditions in which the two heavy and two light chains associate randomly, a hybridoma would make 10 distinct Ig molecules, and the specific antibody would comprise only 1/16 of the total Ig4,5. To obtain hybridomas making only the specific antibodies requires a tumour cell fusion partner that itself makes no Ig but which can nevertheless be fused with spleen cells to obtain hybrids secreting only the specific antibody. We report here the identification of such a cell line, Sp2/0-Ag14.