Systemic Lupus Erythematosus (SLE) Risk Factors: Novel Proteins Detected From Familial SLE Using Proteomics
Systemic Lupus Erythematosus (SLE) Risk Factors: Novel Proteins Detected From Familial SLE Using Proteomics
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DOI：
10.1309/lmiewolo1yldrlhj
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
Yongkang Wu;Zhuochun Huang;Yunying Shi;B. Cai;Lanlan Wang;B. Ying;Chao-jun Hu;Yongzhe Li;W. Liang
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文献类型：
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作者：
Yongkang Wu;Zhuochun Huang;Yunying Shi;B. Cai;Lanlan Wang;B. Ying;Chao-jun Hu;Yongzhe Li;W. Liang
目的探讨蛋白质在系统性红斑狼疮（SLE）发病中的作用。方法应用MALDI-TOF-MS技术对一个SLE家系153份血清标本、63例SLE患者和83名健康对照进行蛋白质组学分析。结果SLE家系中m/z为9342.23的蛋白质水平显著高于SLE患者和健康对照，SLE患者中m/z为9342.23的蛋白质水平显著高于健康对照。这些额外的蛋白质可能是新的多肽，而不是以前检测到的自身抗体或其他蛋白质。SLE家族中m/z为4094.03、5905.35和7973.53的蛋白质明显低于单个SLE患者和健康对照，并且单个SLE中的蛋白质明显低于健康对照。结论4种蛋白质在SLE的发病机制中起重要作用，并可预测SLE的发病风险。具有更多m/z为9342.23的蛋白质和较少m/z为4094.03、5905.35和7973.53的蛋白质的个体可能具有更高的罹患SLE的风险。
Objective In order to investigate the pathogenesis role of proteins in systemic lupus erythematosus (SLE). Methods Protein profiles of 153 serum samples from an SLE family with 7 members, 63 individual SLE patients, and 83 healthy controls were analyzed using MALDI-TOF-MS. Results The protein level at m/z of 9342.23 was significantly higher in the SLE family compared with individual SLE patients and healthy controls, and the protein in individual SLE was significantly higher than healthy controls. The extra proteins are possibly novel peptides instead of autoantibodies or other proteins detected before. Proteins at m/z of 4094.03, 5905.35, and 7973.53 in the SLE family were significantly lower than individual SLE patients and healthy controls, and those in individual SLE were significantly lower than healthy controls. Conclusion The 4 discriminative proteins may play an important role in the pathogenesis of SLE and predict the risk of suffering SLE. Individuals with more protein at an m/z of 9342.23 and less proteins of 4094.03, 5905.35, and 7973.53 may have a higher risk of suffering SLE.