AIE Featured Inorganic-Organic Core@Shell Nanoparticles for High-Efficiency siRNA Delivery and Real-Time Monitoring
AIE Featured Inorganic-Organic Core@Shell Nanoparticles for High-Efficiency siRNA Delivery and Real-Time Monitoring
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AIE 特色无机-有机核@壳纳米粒子，用于高效 siRNA 传输和实时监测
DOI：
10.1021/acs.nanolett.8b04677
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发表时间：
2019-04-01
期刊：
影响因子：
10.8
通讯作者：
Tang, Ben Zhong
中科院分区：
文献类型：
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作者：
He, Xuewen;Yin, Feng;Tang, Ben Zhong
RNA干扰(RNAi)被证明是疾病治疗中最有效的序列特异性基因抑制技术之一。人们一直在探索高效、低细胞毒性和自我监测功能的小干扰RNA(SiRNA)递送的新型载体。在这里，我们利用聚集诱导发射发光剂(AIEgen)的优势，开发了一类新型的银@AIE核壳纳米载体，具有可调节的、均匀的形貌。它在siRNA传递、靶基因敲除和体外肿瘤细胞抑制方面表现出极高的效率。更重要的是，在小动物实验中，抗癌效果高达75%，没有明显的毒性。由于AIE的独特性质，成功地实现了siRNA传递的实时细胞内跟踪和长期的肿瘤组织成像。与商业化的转染剂相比，这种纳米载体在生物相容性、传递效率和重复性方面都有了显著的改善，在RNAi相关的癌症治疗中具有广阔的前景。
RNA interference (RNAi) is demonstrated as one of the most powerful technologies for sequence-specific suppression of genes in disease therapeutics. Exploration of novel vehicles for small interfering RNA (siRNA) delivery with high efficiency, low cytotoxicity, and self-monitoring functionality is persistently pursued. Herein, by taking advantage of aggregation-induced emission luminogen (AIEgen), we developed a novel class of Ag@AIE core@shell nanocarriers with regulable and uniform morphology. It presented excellent efficiencies in siRNA delivery, target gene knockdown, and cancer cell inhibition in vitro. What's more, an anticancer efficacy up to 75% was achieved in small animal experiments without obvious toxicity. Attributing to the unique AIE properties, real-time intracellular tracking of siRNA delivery and long-term tumor tissue imaging were successfully realized. Compared to the commercial transfection reagents, significant improvements were obtained in biocompatibility, delivery efficiency, and reproducibility, representing a promising future of this nanocarrier in RNAi-related cancer therapeutics.