Assessment of periodontal healing by seeding of fibroblast-like cells derived from regenerated periodontal ligament in artificial furcation defects in a dog:: A pilot study
Assessment of periodontal healing by seeding of fibroblast-like cells derived from regenerated periodontal ligament in artificial furcation defects in a dog:: A pilot study
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DOI：
10.1089/107632702753725030
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发表时间：
2002-04-01
影响因子：
--
通讯作者：
Oygür, T
中科院分区：
文献类型：
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作者：
Dogan, A;Özdemir, A;Oygür, T
由于疾病而丢失的牙周支持组织的再生可以通过在暴露的牙根表面重新填充来自牙周膜的细胞来完成。因此，我们的目标是评估再生牙周膜(RPL)来源的细胞的种植以促进犬人工牙根分叉缺损处的再生。将RPL组织的外植体置于聚四氟乙烯(E-PTFE)膜下孵育，获得成纤维细胞样细胞。在下颌第二、第四前磨牙上诱导出II类分叉缺陷。对照缺陷也包括在对侧。将第4代细胞(2×10(5)细胞)悬液置于0.5mL自体血液凝固液中，覆盖每个分叉区。术后第42天对愈合情况进行组织形态计量学评价，并以百分比表示。种植细胞的缺损区有75%的愈合是通过新生的结缔组织附着和牙骨质形成，而对照组是71%。骨形成被发现填补了51%的分叉缺损；然而，在对照部位，它是35%的缺损处。在这项初步研究中，我们认为通过细胞种植技术再生分叉缺陷可能是有用的，但还需要进一步的研究来确定该过程的结果。
The regeneration of periodontal supporting tissues lost as a result of disease could be accomplished by repopulating the exposed root surfaces with cells originating from periodontal ligament. Thus, we aimed to assess the seeding of cells derived from regenerated periodontal ligament (RPL) to promote the regeneration in artificial furcation defects of a dog. The fibroblast-like cells were obtained by incubating the explants of RPL tissue taken under a teflon (E-PTFE) membrane. Class II furcation defects were induced on the second and fourth mandibular premolars. Control defects were also included on the contralateral side. A suspension of the fourth passage cells (2 x 10(5) cells) in 0.5 mL of autologous blood coagulum was placed over each furcation area. The healing was histomorphometrically evaluated at the 42nd day postoperatively and expressed as percentage. The healing by new connective tissue attachment with cementum formation was found 75% in the cell-seeding defects whereas, it was 71% in controls. Bone formation was found to fill 51% of furcation defects; however, it was 35% of the defects in the control sites. In this pilot study, we suggested that regeneration of furcation defects by cell-seeding technique may be useful, but further studies are needed to determine the outcome of the procedure.