THE MARINER TRANSPOSABLE ELEMENT IN THE DROSOPHILIDAE FAMILY
THE MARINER TRANSPOSABLE ELEMENT IN THE DROSOPHILIDAE FAMILY
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DOI：
10.1038/hdy.1994.185
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发表时间：
1994-10-01
期刊：
影响因子：
3.8
通讯作者：
CAPY, P
中科院分区：
文献类型：
--
作者：
BRUNET, F;GODIN, F;CAPY, P
利用两组引物（一组对应于倒置末端重复序列，另一组对应于推测转座酶的两个保守区域），采用三种不同的技术（即squash blots、Southern blots和PCR扩增）研究了果蝇mariner转座因子在物种中的分布。我们的结果和其他人的结果表明，水手的分布是不均匀的，不遵循宿主物种的系统发育。基于特有物种的地理分布分析表明，水手主要存在于亚洲和非洲。至少可以提出两种假设来解释这种元素的具体地理分布。首先，它们可能是果蝇物种之间和/或果蝇物种与果蝇科以外的一个或几个供体物种之间多次水平传播的结果。其次，这些特殊的分布可能对应于果蝇科祖先中存在的祖先副本的水手元素的进化。
The distribution of the mariner transposable element among Drosophilidae species was investigated using three different techniques, i.e. squash blots, Southern blots and PCR amplification, using two sets of primers (one corresponding to the Inverted Terminal Repeats and the other to two conserved regions of the putative transposase). Our results and those of others show that the distribution of mariner is not uniform and does not follow the phylogeny of the host species. An analysis of geographical distribution, based on endemic species, shows that mariner is mainly present in Asia and Africa. At least two hypotheses may be proposed to explain the specific and geographical distributions of this element. Firstly, they may be the results of several horizontal transmissions between Drosophila species and/or between Drosophila species and one or several donor species outside the Drosophilidae family. Secondly, these particular distributions may correspond to the evolution of the mariner element from an ancestral copy which was present in the ancestor of the Drosophilidae family.