Nonyophenol reduced the number of haploids in in vitro spermatogenesis of the endangered cyprinid Gnathopogon caerulescens
Nonyophenol reduced the number of haploids in in vitro spermatogenesis of the endangered cyprinid Gnathopogon caerulescens
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壬基苯酚减少了濒危鲤鱼 Gnathopogon caerulescens 体外精子发生中单倍体的数量
DOI：
10.1016/j.tiv.2023.105565
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发表时间：
2023
影响因子：
3.2
通讯作者：
Takada T.
中科院分区：
文献类型：
--
作者：
Nishie T.;Komaru. A.;Shiroguchi S.;Yamaizumi T.;Ono Y.;Motomochi A.;Tooyama I.;Fujioka Y.;Sakai N.;Higaki S.;Takada T.
壬基酚（NP）是一种内分泌干扰物，广泛应用于工农业生产过程中，导致NP进入水环境。NP诱导激素失衡和男性女性化，并减少精子发生过程中生殖细胞的产生;然而，它影响精子发生的机制仍然未知。在这里，我们研究了NP对精子发生的影响honmoroko（Gnathopogon caerulescens），一种濒危鱼类特有的日本琵琶湖，使用一个体外分化系统。我们采集了未产卵的雄鹅睾丸中的精原细胞。caerulescens并进行悬浮培养。无论NP的存在与否，精原细胞在3周内分化为鞭毛精子。低至1 nM的NP浓度以剂量依赖性方式导致生殖细胞数量减少，而体细胞数量仅在1 μM的高浓度下减少。流式细胞仪分析表明，生殖细胞数量的减少是由于单倍体（精子细胞和精子），精原细胞和精母细胞的数量不受NP治疗。这一结果与NP可能抑制单倍体第二次减数分裂或诱导细胞凋亡的假说一致。本研究表明，体外生殖细胞分化和流式细胞术分析相结合，是有用的评估的直接影响NP对生殖细胞分化的濒危特有鱼类。
Nonylphenol (NP), an endocrine disrupting chemical, is widely used in industrial and agricultural processes, causing NP influx into aquatic environments. NP induces hormonal imbalance, and male feminization, and reduces germ cell production during spermatogenesis; however, the mechanism by which it affects spermatogenesis remains unknown. Here, we investigated the effect of NP on spermatogenesis in honmoroko (Gnathopogon caerulescens), an endangered fish endemic to Lake Biwa, Japan, using anin vitrodifferentiation system. We collected spermatogonia from the testes of non-spawningG. caerulescensand subjected them to suspension culture. The spermatogonia differentiated into flagellated spermatozoa in 3 weeks, regardless of the presence of NP. NP concentrations as low as 1 nM caused a decrease in the number of germ cells in a dose-dependent manner, whereas the number of somatic cells decreased only at a high concentration of 1 μM. Flow cytometric analysis revealed that the decrease in germ cell number was attributed to haploids (spermatids and spermatozoa); the number of spermatogonia and spermatocytes was not affected by NP treatment. This result is consistent with the hypothesis that NP might repress the second meiosis or induce apoptosis in haploids. This study demonstrated that the combination ofin vitrogerm cell differentiation and flow cytometric analysis is useful for evaluating the direct effects of NP on germ cell differentiation in endangered endemic fish.