Postischemic accumulation of lipid peroxidation products in the rat brain: immunohistochemical detection of 4-hydroxy-2-nonenal modified proteins
Postischemic accumulation of lipid peroxidation products in the rat brain: immunohistochemical detection of 4-hydroxy-2-nonenal modified proteins
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大鼠脑缺血后脂质过氧化产物的积累：4-羟基-2-壬烯醛修饰蛋白的免疫组织化学检测
DOI：
10.1016/s0006-8993(97)00616-1
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发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
Y. Mizuno
中科院分区：
文献类型：
--
作者：
H. Yoshino;N. Hattori;T. Urabe;K. Uchida;Masashi Tanaka;Y. Mizuno
我们报告的分布和4-羟基-2-壬烯醛（HNE）修饰的蛋白质，脂质过氧化反应的指标，在大鼠大脑中动脉（MCA）闭塞3小时后再灌注的免疫组织化学研究。HNE免疫反应在完整的神经元中未观察到，但在再灌注后3 h，在梗死区的一些萎缩的神经元中出现。HNE阳性神经元的数量随着梗死面积的扩大而增加。额顶叶皮质第三层锥体神经元呈HNE阳性反应，再灌注后24 h其HNE免疫反应强度最高。在48小时，HNE阳性神经元观察到的内侧部分的纹状体，外侧的额顶叶皮质，并在梗死区和非梗死区之间的边界。此外，在小胶质细胞中观察到强HNE免疫反应性（通过OX-42免疫染色鉴定）。这种方法似乎是有用的，以跟踪缺血损伤后的脂质过氧化反应在细胞水平上的进展。
We report an immunohistochemical study on the distribution and alterations of 4-hydroxy-2-nonenal (HNE)-modified proteins, an indicator of lipid peroxidation, in the rat brain after 3 h of middle cerebral artery (MCA) occlusion followed by reperfusion. HNE immunoreactivity was not observed in intact neurons, but it appeared in some shrunken neurons within the infarcted zone at 3 h after reperfusion. The number of HNE-positive neurons increased with the spread of the infarcted area. The pyramidal neurons in the third layer of the frontoparietal cortex were HNE-positive and the intensity of their HNE immunoreactivity was highest at 24 h after reperfusion. At 48 h, HNE-positive neurons were observed in the medial part of the striatum, the lateral side of the frontoparietal cortex, and at the boundary between the infarcted and noninfarcted zones. In addition, strong HNE immunoreactivity was seen in microglia (identified by OX-42 immunostaining). This method seems to be useful to follow the progress of lipid peroxidation at the cellular level after ischemic injury.
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抗氧化剂减少过氧化氢介导的线粒体转录抑制。
影响因子：
7.4
作者：
Kristal,BS;Park,BJ;Yu,BP
通讯作者：
Yu,BP
DOI：
10.1073/pnas.87.13.5144
发表时间：
1990-07-01
影响因子：
11.1
作者：
OLIVER, CN;STARKEREED, PE;FLOYD, RA
通讯作者：
FLOYD, RA
影响因子：
1.7
作者：
CHEN, J;GRAHAM, SH;SIMON, RP
通讯作者：
SIMON, RP
DOI：
10.1161/01.atv.10.3.336
发表时间：
1990-05-01
期刊：
ARTERIOSCLEROSIS
影响因子：
--
作者：
ROSENFELD, ME;PALINSKI, W;WITZTUM, JL
通讯作者：
WITZTUM, JL