The F-box family genes as key elements in response to salt, heavy mental, and drought stresses in Medicago truncatula
The F-box family genes as key elements in response to salt, heavy mental, and drought stresses in Medicago truncatula
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DOI：
10.1007/s10142-015-0438-z
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发表时间：
2015-04
影响因子：
2.9
通讯作者：
J. Song;Yan Wang;Hai Bo Li;B. Li;Z. Zhou;Shuai Gao;Z. Yang
中科院分区：
文献类型：
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作者：
J. Song;Yan Wang;Hai Bo Li;B. Li;Z. Zhou;Shuai Gao;Z. Yang
F-box蛋白是Skp1-Rbx1-Cul1-F-box蛋白（SCF）复合物的一个亚基，具有约40个氨基酸的典型保守的F-box基序，是真核生物中最大的蛋白家族之一。F-box蛋白通过26S蛋白酶体在选择性和特异性蛋白降解中起关键作用。在这项研究中，我们用生物信息学方法从紫花苜蓿基因组中鉴定了972个推测的F-box蛋白。我们的分析表明，除了保守基序外，F-box蛋白在其c端区域还具有其他几个功能域（例如LRRs， Kelch， FBA和PP2），其中一些被发现为m。truncatulaspecies-specific。通过对F-box基序的系统发育分析，这些蛋白可分为三个主要家族，每个家族又可进一步分为多个亚群。m -box基因的基因组分布分析。截断染色体揭示了m -box基因的进化扩增。截尾可能是由于局部基因重复造成的。为了研究F-box基因对非生物胁迫的可能反应，我们分析了公开可用的和客户制备的微阵列。大多数F-box蛋白基因可以对盐和重金属胁迫做出反应。Real-time PCR分析证实，F-box蛋白中部分含有热、干旱、水杨酸和脱落酸响应元件的基因能够对非生物胁迫做出响应。
F-box protein is a subunit of Skp1-Rbx1-Cul1-F-box protein (SCF) complex with typically conserved F-box motifs of approximately 40 amino acids and is one of the largest protein families in eukaryotes. F-box proteins play critical roles in selective and specific protein degradation through the 26S proteasome. In this study, we bioinformatically identified 972 putative F-box proteins fromMedicago truncatulagenome. Our analysis showed that in addition to the conserved motif, the F-box proteins have several other functional domains in their C-terminal regions (e.g., LRRs, Kelch, FBA, and PP2), some of which were found to beM. truncatulaspecies-specific. By phylogenetic analysis of the F-box motifs, these proteins can be classified into three major families, and each family can be further grouped into more subgroups. Analysis of the genomic distribution of F-box genes onM. truncatulachromosomes revealed that the evolutional expansion of F-box genes inM. truncatulawas probably due to localized gene duplications. To investigate the possible response of the F-box genes to abiotic stresses, both publicly available and customer-prepared microarrays were analyzed. Most of the F-box protein genes can be responding to salt and heavy metal stresses. Real-time PCR analysis confirmed that some of the F-box protein genes containing heat, drought, salicylic acid, and abscisic acid responsivecis-elements were able to respond to the abiotic stresses.