Regulation of chemokine production via oxidative pathway in HeLa cells.
Regulation of chemokine production via oxidative pathway in HeLa cells.
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DOI：
10.1155/2009/183760
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发表时间：
2009
影响因子：
4.6
通讯作者：
Sunakawa H
中科院分区：
文献类型：
--
作者：
Kina S;Nakasone T;Takemoto H;Matayoshi A;Makishi S;Sunagawa N;Liang F;Phonaphonh T;Sunakawa H
炎症与疾病进展相关，并且据说通过很大程度上未知的机制驱动肿瘤发生。在炎症部位，中性粒细胞部署了一个有效的抗菌武器库，包括蛋白酶，抗菌肽和ROS。活性氧（ROS）诱导趋化因子。采用酶联免疫吸附法（ELISA）检测活性氧（ROS）作用后HeLa细胞培养上清中IL-8的浓度。我们使用邻菲咯啉耗尽Fe 2+，以研究ROS诱导IL-8分泌的机制。 铁螯合剂邻菲咯啉能有效抑制H2 O2诱导的ERK 2活化。酶联免疫吸附试验表明，IL-8蛋白的分泌升高ROS处理的HeLa细胞。当从这些细胞中去除Fe 2+时，IL-8分泌被抑制。总的来说，这些结果表明，Fe 2+介导的Erk途径激活是ROS诱导的上皮细胞IL-8分泌的重要信号转导途径。
Inflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis. At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS. Reactive oxygen species (ROSs) induce chemokines. In the present study, the concentrations of IL-8 in culture supernatants of HeLa cells treated with ROS were determined by enzyme-linked immunosorbent assay. We used o-phenanthroline to deplete Fe2+ in order to investigate the mechanisms through which ROSs induce IL-8 secretion in our system. The iron chelator o-phenanthroline effectively inhibited H2O2-induced ERK2 activation. Enzyme-linked immunosorbent assays showed that IL-8 protein secretion was elevated in ROS-treated HeLa cells. When Fe2+ was removed from these cells, IL-8 secretion was inhibited. Collectively, these results indicate that Fe2+-mediated Erk pathway activation is an important signal transduction pathway in ROS-induced IL-8 secretion in epithelial cells.
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影响因子：
4.8
作者：
Guyton, KZ;Liu, YS;Holbrook, NJ
通讯作者：
Holbrook, NJ
影响因子：
4.6
作者：
Méndez-Samperio P;Palma-Barrios J;Vázquez-Hernández A;García-Martínez E
通讯作者：
García-Martínez E
影响因子：
82.9
作者：
Mizukami, Y;Jo, WS;Chung, DC
通讯作者：
Chung, DC
影响因子：
16
作者：
Hara, Y;Wakamori, M;Mori, Y
通讯作者：
Mori, Y
影响因子：
4
作者：
Choi, Eun-Young;Park, Zee-Yong;Jun, Chang-Duk
通讯作者：
Jun, Chang-Duk