In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio)
In vivo studies of liver-type fatty acid binding protein (L-FABP) gene expression in liver of transgenic zebrafish (Danio rerio)
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DOI：
10.1016/s0014-5793(03)00157-1
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发表时间：
2003-03-13
期刊：
影响因子：
3.5
通讯作者：
Wu, JL
中科院分区：
文献类型：
--
作者：
Her, GM;Chiang, CC;Wu, JL
哺乳动物肝脏脂肪酸结合蛋白(L-FABP)是一种存在于肝脏、小肠和肾脏等多种组织中的小分子胞浆蛋白，被认为在细胞内脂肪酸的运输和代谢中起着至关重要的作用。为了更好地了解其在斑马鱼肝脏发生过程中的组织特异性调控，我们分离了斑马鱼L-FABP基因的5‘端序列，并采用绿色荧光蛋白转基因策略获得了肝脏特异表达的转基因斑马鱼。斑马鱼L-FABP基因5‘侧翼2.8kb的序列足以引导绿色荧光蛋白在肝脏原基中表达，首先在2个斑马鱼胚胎中观察到绿色荧光蛋白，然后持续到成鱼阶段。这种转基因表达模式与内源基因的表达模式是一致的。7个转基因品系的F2遗传率在42~51%之间，符合孟德尔分离比例。此外，HHEX和zXBP-1变异体显示出明显的肝脏缺陷，这表明建立一个体内系统来筛选肝脏发育所需的基因是可能的。(C)2003年，埃尔塞维尔科学公司代表欧洲生化学会联合会出版。
Mammalian liver fatty acid binding protein (L-FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue-specific regulation during zebrafish hepatogenesis, we isolated 5'-flanking sequences of the zebrafish L-FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver-specific transgenic zebrafish. The 2.8-kb 5'-flanking sequence of zebrafish L-FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42-51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp-1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.