AXONLESS HORIZONTAL CELLS OF THE RABBIT RETINA - SYNAPTIC CONNECTIONS AND ORIGIN OF THE ROD AFTEREFFECT
AXONLESS HORIZONTAL CELLS OF THE RABBIT RETINA - SYNAPTIC CONNECTIONS AND ORIGIN OF THE ROD AFTEREFFECT
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DOI：
10.1007/bf01188041
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发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
DACHEUX, RF
中科院分区：
文献类型：
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作者：
RAVIOLA, E;DACHEUX, RF
用微电极穿透兔视网膜无轴突水平细胞（AHC），记录其光反应后，用辣根过氧化物酶染色。然后将细胞连续切片，并用电子显微镜检查其连接。生理上，细胞表现出锥主导的反应和一个小杆的影响，杆后效。电子显微镜显示，该AHC只连接到锥。因此，视杆后效只能通过视杆细胞和视锥细胞突触末梢之间的差距连接侵入细胞。在与细胞接触的锥体蒂的突触内陷中，只有一个外侧元件被染色。这表明每个锥体内陷突触的两个侧成分属于两个不同的水平细胞。通过对细胞内相邻的AHC进行染色，我们发现两个侧突可能起源于属于同一形态类型的两个水平细胞。
An axonless horizontal cell (AHC) of the rabbit retina was penetrated with a microelectrode and stained with horseradish peroxidase after recording its light responses. The cell was then serially sectioned and its connections examined with the electron microscope. Physiologically, the cell exhibited cone-dominated responses and a minor rod influence known as rod aftereffect. Electron microscopy showed that this AHC was only connected to cones. Therefore, the rod aftereffect could only invade the cell through the gap junctions between the synaptic endings of rod and cone photoreceptors. In the synaptic invaginations of the cone pedicles contacting the cell, only one of the lateral elements was stained. This suggests that the two lateral elements of each cone-invaginating synapse belong to two different horizontal cells. By staining intracellularly adjacent AHCs, we showed that the two lateral processes may originate from two horizontal cells belonging to the same morphological type.