Multicolor enzyme-linked immunosorbent sensor for sensitive detection of organophosphorus pesticides based on TMB2+-mediated etching of gold nanorods

Multicolor enzyme-linked immunosorbent sensor for sensitive detection of organophosphorus pesticides based on TMB2+-mediated etching of gold nanorods
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基于TMB2介导的金纳米棒蚀刻的多色酶联免疫吸附传感器,用于灵敏检测有机磷农药

DOI:
10.1016/j.microc.2021.106411
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发表时间:
2021-09
影响因子:
4.8
通讯作者:
Yu Zhou
Yu Zhou
中科院分区:
化学2区
文献类型:
--
作者:
Xiao-Li Yin;Yun Qing Liu;Hui-Wen Gu;Qi Zhang;Zhao Wei Zhang;Hui Li;Pei-Wu Li;Yu Zhou

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A multicolor enzyme-linked immunosorbent sensor was constructed to detect organophosphates pesticides (OPs) on base of TMB2+-mediated AuNRs etching. As the etching progresses, a rainbow-like color responses was generated. The naked eye limit of detection (LOD) of the sensing system is 0.25 ng mL−1. In particular, the longitudinal localized surface plasmon resonances (LSPR) peak shift of AuNRs is linearly correlated with the logarithm of OPs concentration in range of 104to 5 × 106pg mL−1for parathion-methyl and fenitrothion, 5 × 104to 107pg mL−1for fenthion, respectively. Compared with reported methods, this sensor displayed satisfactory linear ranges and detection limits. Average recoveries of this method ranged from 73.28% to 98.08% and relative standard deviations (RSDs) were below 8.0% as for food samples analysis without complicated pretreatments. Remarkably, the developed multicolor enzyme-linked immunosorbent sensor is capable of providing accurate and reliable assay for OPs in complex food samples.
丙酸酯胆碱酯酶:进化上古老的酶的体外表征研究有机磷的农药毒性和重新激活。
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