A comparison of reversible versus irreversible protein glutathionylation.
A comparison of reversible versus irreversible protein glutathionylation.
复制标题
DOI：
10.1016/b978-0-12-420117-0.00005-0
复制
发表时间：
2014
影响因子：
--
通讯作者：
Cooper AJ
中科院分区：
文献类型：
--
作者：
Townsend DM;Lushchak VI;Cooper AJ
谷胱甘肽化通常是一种可逆的翻译后修饰，其发生于已暴露于活性氧的半胱氨酸残基（P-SSG）。这种循环过程可以调节各种蛋白质簇，包括那些参与关键细胞信号功能的蛋白质。然而，某些条件可以有利于脱氢氨基酸的形成，例如2，3-二脱氢丙氨酸（2，3-脱氢丙氨酸，DHA）和2，3-二脱氢丁酸（2，3-脱氢丁酸），其可以充当迈克尔受体。反过来，这些可以与谷胱甘肽（GSH）形成迈克尔加合物，导致形成稳定的硫醚缀合物，这是一种称为不可还原谷胱甘肽化的不可逆过程。预计这在自然界中是普遍存在的，特别是在蛋白质转换较慢的情况下。这种不可还原的谷胱甘肽化可以与更容易的循环信号传导过程区分开来，并且预计具有老年学、毒理学、药理学和肿瘤学相关性。在这里，我们比较可逆和不可逆谷胱甘肽化。
Glutathionylation is generally a reversible posttranslational modification that occurs to cysteine residues that have been exposed to reactive oxygen species (P-SSG). This cyclical process can regulate various clusters of proteins, including those involved in critical cellular signaling functions. However, certain conditions can favor the formation of dehydroamino acids, such as 2,3-didehydroalanine (2,3-dehydroalanine, DHA) and 2,3-didehydrobutyrine (2,3-dehydrobutyrine), which can act as Michael acceptors. In turn, these can form Michael adducts with glutathione (GSH), resulting in the formation of a stable thioether conjugate, an irreversible process referred to as nonreducible glutathionylation. This is predicted to be prevalent in nature, particularly in more slowly turning over proteins. Such nonreducible glutathionylation can be distinguished from the more facile cycling signaling processes and is predicted to be of gerontological, toxicological, pharmacological, and oncological relevance. Here, we compare reversible and irreversible glutathionylation.