C. elegans neurons have functional dendritic spines
C. elegans neurons have functional dendritic spines
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DOI：
10.7554/elife.47918
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发表时间：
2019-10-04
期刊：
影响因子：
7.7
通讯作者：
Miller, David M., III
中科院分区：
文献类型：
--
作者：
Cuentas-Condori, Andrea;Ben Mulcahy;Miller, David M., III
树突棘是一种特殊的突触后结构，负责传递突触前信号，受神经活动的调节，与学习和记忆有关。大多数关于脊柱功能的研究都集中在哺乳动物的神经系统上。然而，在C.线虫（Philbrook等，2018），这表明可以利用较小的模式生物的实验优势来研究树突棘的生物学。在这里，我们使用超分辨率显微镜，电子显微镜，活细胞成像和遗传学，以表明C。线虫运动神经元具有功能性树突棘，其：（1）在结构上由动态肌动蛋白细胞骨架限定;（2）并列突触前致密投射;（3）定位ER和核糖体;（4）显示由突触前活动触发并由内部Ca++储存传播的钙瞬变;（5）响应调节棘密度的活动依赖性信号。这些研究为开发C语言的能力提供了一个新的实验范例。elegans遗传学和活细胞成像用于树突棘形态发生和功能的基础研究。
Dendritic spines are specialized postsynaptic structures that transduce presynaptic signals, are regulated by neural activity and correlated with learning and memory. Most studies of spine function have focused on the mammalian nervous system. However, spine-like protrusions have been reported in C. elegans (Philbrook et al., 2018), suggesting that the experimental advantages of smaller model organisms could be exploited to study the biology of dendritic spines. Here, we used super-resolution microscopy, electron microscopy, live-cell imaging and genetics to show that C. elegans motor neurons have functional dendritic spines that: (1) are structurally defined by a dynamic actin cytoskeleton; (2) appose presynaptic dense projections; (3) localize ER and ribosomes; (4) display calcium transients triggered by presynaptic activity and propagated by internal Ca++ stores; (5) respond to activity-dependent signals that regulate spine density. These studies provide a solid foundation for a new experimental paradigm that exploits the power of C. elegans genetics and live-cell imaging for fundamental studies of dendritic spine morphogenesis and function.