Red blood cell hemolysis during blood bank storage: using national quality management data to answer basic scientific questions
Red blood cell hemolysis during blood bank storage: using national quality management data to answer basic scientific questions
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DOI：
10.1111/j.1537-2995.2009.02275.x
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发表时间：
2009-12-01
期刊：
影响因子：
2.9
通讯作者：
Devine, Dana V.
中科院分区：
文献类型：
--
作者：
Hess, John R.;Sparrow, Rosemary L.;Devine, Dana V.
背景：血库储存过程中红细胞的溶血是红细胞储存系统失效的最明显表现。然而，由于单位间差异的最大来源是捐助方特有的，因此很难对其进行分析。从国家血液系统的数据，用于内部质量控制（QC）的目的，并存储和处理在统一的方式允许statistical analysis.Study设计和方法：溶血措施期间和结束时，随机选择的捐助单位观察QC的目的，从四个国家血液系统。这些措施，从单位，经历了类似的处理和存储组进行了排序，创建直方图和直方图进行了比较statistics.Results：共获得14,087措施下7个存储条件，其中包括超过12,000个措施，在一个国家在四个密切相关的条件。溶血百分比的分布为偏态正态分布，离群值为随机分布。添加剂溶液似乎是等效的，除了AS-1中的42 mmol/L甘露醇与盐水、腺嘌呤、葡萄糖和甘露醇中的传统30 mmol/L甘露醇相比可以减少溶血。从35天增加到42天的储存增加了30%的测定溶血和白细胞减少减少了53%.CONCLUSION：大型国家数据集提供了有用的信息，溶血的分布在RBC储存结束。这些信息可以帮助血液储存系统的开发和监管科学。
BACKGROUND: Hemolysis of red blood cells (RBCs) during blood bank storage is the most obvious manifestation of RBC storage system failure. However, its analysis is made difficult because the largest source of interunit difference is donor specific. Availability of data from national blood systems on large numbers of RBC units used for internal quality control (QC) purposes and stored and processed in uniform ways permits statistical analysis.STUDY DESIGN AND METHODS: Measures of hemolysis during and at the end of storage on randomly selected donor units observed for QC purposes were obtained from four national blood systems. Groups of these measures from units that had undergone similar processing and storage were sorted to create histograms and the histograms were compared statistically.RESULTS: A total of 14,087 measures were obtained under seven storage conditions, including more than 12,000 measures made in a single country under four closely related conditions. Distributions of percent hemolysis are skewed normal and outliers are random. Additive solutions appear to be equivalent, except that the 42 mmol/L mannitol in AS-1 reduces hemolysis compared to conventional 30 mmol/L mannitol in saline, adenine, glucose, and mannitol. Increasing storage from 35 to 42 days increased measured hemolysis by 30% and leukoreduction decreased it by 53%.CONCLUSIONS: Large national data sets provide useful information about the distribution of hemolysis at the end of RBC storage. This information can aid blood storage system development and regulatory science.