Wide mutational spectrum of a gene involved in hormone action and insecticide resistance in Drosophila melanogaster.
Wide mutational spectrum of a gene involved in hormone action and insecticide resistance in Drosophila melanogaster.
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果蝇激素作用和杀虫剂抗性相关基因的广泛突变谱。
DOI：
10.1007/s00438-006-0138-4
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Farkas,Robert
中科院分区：
文献类型：
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作者：
Wilson,ThomasG;Wang,Shaoli;Beno,Milan;Farkas,Robert
黑腹果蝇（drosophila melanogasteria）的耐甲氧二丁烯（methoprene - resistant, Met） bHLH-PAS基因参与幼代激素（JH）的分子作用，突变体对JH和JH激动剂杀虫剂（如甲氧二丁烯）的毒性和形态发生作用产生抗性。对突变体的详细研究可以揭示对JH知之甚少的作用以及对JH杀虫剂抗药性的分子基础。我们检测了9个携带点突变的突变等位基因在3个表型性状上的外显率和表达性：抗性、卵发生缺陷和一种新的眼睛缺陷。收集的等位基因范围从两个表型不太严重的弱等位基因到具有与空等位基因相似的严重表型的强等位基因。突变点位于保守结构域和非保守结构域。复眼后缘严重畸形的眼缺陷和卵子发生表型都是无条件的，而抗性表型的表达需要在早期变态期间用JH或JH类似物（JHAs）治疗。所有表型特征的基础都集中在MET作为蜕变过程中蜕皮激素次级反应靶基因的转录调节剂的作用上。在早期变态过程中，由于突变或JHA的存在而导致MET功能的破坏，导致不同靶基因的转录失调，从而导致两种情况下的病理。导致抗性的MET氨基酸变化的多样性可能预示着随着田间昆虫种群中JH杀虫剂的使用增加，抗性会迅速上升。
TheMethoprene-tolerant(Met) bHLH-PAS gene inDrosophila melanogasteris involved in the molecular action of juvenile hormone (JH), and mutants result in resistance to the toxic and morphogenetic effects of JH and JH agonist insecticides such as methoprene. A detailed study ofMetmutants can shed light on the poorly understood action of JH as well as the molecular basis ofMetresistance to JH insecticides. Nine mutant alleles bearing point mutations inMetwere examined for penetrance and expressivity of three phenotypic characters: resistance, defective oogenesis, and a novel eye defect. The collection ranged from two weak alleles having less severe phenotypes to strong alleles with severe phenotypes similar to that of a null allele. The point mutations were located in both conserved and nonconserved domains. Both the eye defect, seen as severely malformed ommatidial facets in the posterior margin of the compound eye, and the oogenesis phenotype are nonconditional, whereas expression of the resistance phenotype requires treatment with JH or JH analogs (JHAs) during early metamorphosis. A proposed basis for all the phenotypic characters centers on MET action as a transcriptional regulator of ecdysone secondary-response target genes during metamorphosis. Disruption of MET function either by mutation or by JHA presence during early metamorphosis results in transcriptional misregulation of different target genes, resulting in the pathology seen in either instance. The variety of amino acid changes in MET that resulted in resistance may portend a rapid rise in resistance in response to increased use of JH insecticides in field insect populations.