Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis
Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis
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DOI：
10.1128/iai.71.5.2404-2413.2003
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发表时间：
2003-05-01
影响因子：
3.1
通讯作者：
Drenkard, E
中科院分区：
文献类型：
--
作者：
Miyata, S;Casey, M;Drenkard, E
非脊椎动物模型宿主是研究宿主-病原体相互作用的有价值的工具，因为它们有助于鉴定细菌毒力因子，并允许发现参与宿主先天免疫反应的新组分。在这份报告中，我们确定，大蜡螟毛虫大蜡螟是一个方便的非哺乳动物模式主机的第三型分泌系统（TTSS）在铜绿假单胞菌发病机制的作用的研究。基于铜绿假单胞菌PA 14株TTSS pscD基因突变导致G.为了研究铜绿假单胞菌（P. aeruginosa）的四种已知的效应蛋白（ExoS、ExoT、ExoU和ExoY）在杀死蜡螟中的作用，我们研究了它们在杀死蜡螟中的作用。我们确定在铜绿假单胞菌菌株PA 14中，只有ExoT和ExoU在G.大蜡螟致死。菌株PA 14缺乏ExoS效应蛋白的编码序列，并且似乎不表达ExoY。此外，使用DeltaexoU DeltaexoY、DeltaexoT DeltaexoY和DeltaexoT DeltaexoU双突变体，我们确定ExoT或ExoU的单独易位足以获得接近野生型水平的G。大蜡螟致死。另一方面，用DeltaexoT DeltaexoU DeltaexoY三重突变体和DeltapscD突变体获得的数据表明，另外的、尚未鉴定的铜绿假单胞菌III型分泌物组分参与G.梅隆内拉G. Mellonella模型和用哺乳动物组织培养系统进行的细胞病理学测定的结果验证了G.用于铜绿假单胞菌TTSS的研究。
Nonvertebrate model hosts represent valuable tools for the study of host-pathogen interactions because they facilitate the identification of bacterial virulence factors and allow the discovery of novel components involved in host innate immune responses. In this report, we determined that the greater wax moth caterpillar Galleria mellonella is a convenient nonmammalian model host for study of the role of the type III secretion system (TTSS) in Pseudomonas aeruginosa pathogenesis. Based on the observation that a mutation in the TTSS pscD gene of P. aeruginosa strain PA14 resulted in a highly attenuated virulence phenotype in G. mellonella, we examined the roles of the four known effector proteins of P. aeruginosa (ExoS, ExoT, ExoU, and ExoY) in wax moth killing. We determined that in P. aeruginosa strain PA14, only ExoT and ExoU play a significant role in G. mellonella killing. Strain PA14 lacks the coding sequence for the ExoS effector protein and does not seem to express ExoY. Moreover, using DeltaexoU DeltaexoY, DeltaexoT DeltaexoY, and DeltaexoT DeltaexoU double mutants, we determined that individual translocation of either ExoT or ExoU is sufficient to obtain nearly wild-type levels of G. mellonella killing. On the other hand, data obtained with a DeltaexoT DeltaexoU DeltaexoY triple mutant and a DeltapscD mutant suggested that additional, as-yet-unidentified P. aeruginosa components of type III secretion are involved in virulence in G. mellonella. A high level of correlation between the results obtained in the G. mellonella model and the results of cytopathology assays performed with a mammalian tissue culture system validated the use of G. mellonella for the study of the P. aeruginosa TTSS.