in situ oestrone synthesis in normal breast and breast tumour tissues: Effect of treatment with 4‐hydroxyandrostenedione
in situ oestrone synthesis in normal breast and breast tumour tissues: Effect of treatment with 4‐hydroxyandrostenedione
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正常乳腺和乳腺肿瘤组织中的原位雌酮合成：4-羟基雄烯二酮治疗的效果
DOI：
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发表时间：
1989
影响因子：
6.4
通讯作者：
V. James
中科院分区：
文献类型：
--
作者：
M. Reed;A. Owen;L. Lai;N. Coldham;M. Ghilchik;N. Shaikh;V. James
同位素输注技术已被用于试图确定的贡献，当地，在原位，雌酮的合成使乳腺肿瘤的雌激素含量。3 H-雄烯二酮和14 C-雌酮在手术前12小时输注到晚期乳腺癌患者中。手术时，获得正常乳腺和乳腺肿瘤活检样本，分离并测量3 H-雄烯二酮、源自3 H-雄烯二酮的3 H-雌酮和14 C-雌酮。DNA聚合酶α活性，细胞增殖的标志物，也进行了测量，以检查是否雌酮的局部合成产生的生物学效应。在患者接受芳香酶抑制剂4-羟基雄烯二酮治疗后，在接受进一步手术切除肿瘤之前，重复了该研究。在检查的4/6个肿瘤中，从3 H-雄烯二酮原位合成的3 H-雌酮占检测到的3 H-雌酮的主要部分（84.3 ± 9.0%），而在其他2个肿瘤中未发生显著的原位合成。虽然4-羟基雄烯二酮治疗没有显著改变乳腺组织对3 H-雄烯二酮或14 C-雌酮的摄取，但仅在治疗后的一个肿瘤样本中检测到3 H-雌酮的原位形成。4-羟基雄烯二酮治疗后，4/6例肿瘤的DNA聚合酶α活性降低。然而，总体而言，原位形成的3 H-雌酮水平与DNA聚合酶α活性之间无显著相关性（r = 0.38，NS）。它的结论是，在一些，但不是所有的，乳腺肿瘤原位形成雌酮可以作出重要贡献的雌激素含量的乳腺肿瘤。
An isotopic infusion technique has been used in an attempt to determine the contribution that local, in situ, oestrone synthesis makes to the oestrogen content of breast tumours. 3H‐Androstenedione and 14C‐oestrone were infused into women with advanced breast cancer for 12 hr before operation. At surgery, normal breast and breast tumour biopsy samples were obtained and 3H‐androstenedione, 3H‐oestrone derived from 3H‐androstenedione and 14C‐oestrone were isolated and measured. DNA polymerase α activity, a marker of cellular proliferation, was also measured to examine whether local synthesis of oestrone exerted a biological effect. The study was repeated after patients had been treated with the aromatase inhibitor, 4‐hydroxyandrostenedione, before undergoing further surgery for removal of their tumours. In 4/6 tumours examined, in situ synthesis of 3H‐oestrone from 3H‐androstenedione accounted for the major part (84.3 ± 9.0%) of the 3H‐oestrone detected, while no significant in situ synthesis occurred in 2 other tumours. Although treatment with 4‐hydroxyandrostenedione did not significantly alter the uptake of 3H‐androstenedione or 14C‐oestrone into breast tissues, in situ formation of 3H‐oestrone was only detected in one tumour sample after treatment. DNA polymerase α activity decreased in 4/6 tumours after treatment with 4‐hydroxyandrostenedione. Overall, however, there was no significant correlation between the level of 3H‐oestrone formed in situ and DNA polymerase α activity (r = 0.38, NS). It is concluded that in some, but not all, breast tumours in situ formation of oestrone can make an important contribution to the oestrogen content of breast tumours.
人类乳腺肿瘤中芳香酶活性的生物学意义。
DOI：
10.1210/jcem-57-6-1125
发表时间：
1983
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Tilson-Mallett,N;Santner,SJ;Feil,PD;Santen,RJ
通讯作者：
Santen,RJ