CORRELATION BETWEEN INFECTIVITY AND PHYSICAL VIRUS PARTICLES IN HUMAN CYTOMEGALOVIRUS
CORRELATION BETWEEN INFECTIVITY AND PHYSICAL VIRUS PARTICLES IN HUMAN CYTOMEGALOVIRUS
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DOI：
10.1128/jb.92.5.1555-1561.1966
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
VONKA, V
中科院分区：
文献类型：
--
作者：
BENYESHM.M;PROBSTME.F;VONKA, V
通过沉降假复制技术测定两种不同人巨细胞病毒株的粗品未纯化细胞内制剂的滴度[以空斑形成单位（PFU）计]和颗粒计数。与先前报告的这些病毒的106至108的高颗粒感染率比不同，菌株AD-169的每个PFU的总颗粒数范围为160至490，菌株C-87的范围为176至1，050。根据颗粒形态学对细胞内巨细胞病毒颗粒PFU比的解释目前尚不明确。发现包膜形式的数量在计数的总颗粒的0 - 34%之间变化。然而，真实的比例可能更大，因为发现包膜被用于制备电子显微镜检查标本的酶处理所破坏。发现的完整颗粒数量范围为计数的总颗粒的4%至31%。发现细胞外病毒的颗粒/PFU比率比细胞内病毒低三至五倍。
Infectivity titers [measured as plaque-forming units (PFU)] and particle counts by the sedimentation pseudoreplication technique were determined for crude unpurified intracellular preparations of two different strains of human cytomegalovirus. Unlike the high particle-infectivity ratio of 106 to 108 previously reported for these viruses, the number of total particles per PFU ranged from 160 to 490 with strain AD-169 and from 176 to 1,050 for strain C-87. Interpretation of particle PFU ratios of intracellular cytomegalovirus in terms of particle morphology is not conclusive at this time. The number of enveloped forms found varied between 0 and 34% of the total particles counted. However, the true proportion is probably greater, because envelopes were found to be destroyed by the enzyme treatment used in preparing the specimens for examination in the electron microscope. The number of full particles found ranged between 4 and 31% of the total particles counted. The particle per PFU ratio of extracellular virus was found to be three- to five-fold lower than that of intracellular virus.