Crystalline and amorphous cellulose in the secondary walls of Arabidopsis
Crystalline and amorphous cellulose in the secondary walls of Arabidopsis
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DOI：
10.1016/j.plantsci.2012.05.008
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发表时间：
2012-09-01
期刊：
影响因子：
5.2
通讯作者：
Joseleau, Jean-Paul
中科院分区：
文献类型：
--
作者：
Ruel, Katia;Nishiyama, Yoshiharu;Joseleau, Jean-Paul
在高等植物的细胞壁中，纤维素链以结晶微纤维的形式存在，表面有无定形部分，或者以无定形物质的形式存在。为了评估拟南芥花序茎中两种形式纤维素的分布和相对发生情况，我们使用了两种碳水化合物结合模块 CBM3a 和 CBM28，分别对结晶和无定形纤维素具有特异性，并在 TEM 中进行免疫金检测。两种 CBM 的结合显示出特定的模式，表明纤维素的合成会根据细胞类型产生不同的纤维素结构纳米域。在发育中的细胞壁中，只有 CBM3a 与初始初生壁显着结合，表明纤维素在其沉积开始时处于晶体结构。随着次生壁的发育，两种 CBM 的标记变得更加强烈。横向和纵向切片之间 CBM3a 标记图案的变化似乎与微纤维方向有关，并且在纤维和血管之间存在差异。尽管这两种 CBM 无法描述纤维素微观结构的完整状态，但它们揭示了在细胞壁形成过程中以及使纤维素微观结构适应细胞功能的细胞类型之间纤维素结晶和无定形形式沉积的动态。 (C) 2012 Elsevier Ireland Ltd. 保留所有权利。
In the cell walls of higher plants, cellulose chains are present in crystalline microfibril, with an amorphous part at the surface, or present as amorphous material. To assess the distribution and relative occurrence of the two forms of cellulose in the inflorescence stem of Arabidopsis, we used two carbohydrate-binding modules, CBM3a and CBM28, specific for crystalline and amorphous cellulose, respectively, with immunogold detection in TEM. The binding of the two CBMs displayed specific patterns suggesting that the synthesis of cellulose leads to variable nanodomains of cellulose structures according to cell type. In developing cell walls, only CBM3a bound significantly to the incipient primary walls, indicating that at the onset of its deposition cellulose is in a crystalline structure. As the secondary wall develops, the labeling with both CBMs becomes more intense. The variation of the labeling pattern by CBM3a between transverse and longitudinal sections appeared related to microfibril orientation and differed between fibers and vessels. Although the two CBMs do not allow the description of the complete status of cellulose microstructures, they revealed the dynamics of the deposition of crystalline and amorphous forms of cellulose during wall formation and between cell types adapting cellulose microstructures to the cell function. (C) 2012 Elsevier Ireland Ltd. All rights reserved.