Developmental expression of solute carrier family 26A member 4 (SLC26A4/pendrin) during amelogenesis in developing rodent teeth
Developmental expression of solute carrier family 26A member 4 (SLC26A4/pendrin) during amelogenesis in developing rodent teeth
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DOI：
10.1111/j.1600-0722.2011.00901.x
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发表时间：
2011-12-01
影响因子：
1.9
通讯作者：
DenBesten, Pamela
中科院分区：
文献类型：
--
作者：
Bronckers, Antonius L. J. J.;Guo, Jing;DenBesten, Pamela
成釉细胞需要在釉质晶体的形成过程中调节pH值，这是一个产生质子的过程。溶质载体家族26 A成员4（SLC 26 A4或pendrin）是氯离子、碳酸氢根、碘离子和甲酸根的阴离子交换剂。在肾脏、内耳和甲状腺中离子转运上皮细胞的顶膜中表达，在那里调节管腔pH和液体转运。我们假设成熟成釉细胞表达SLC 26 A4以中和釉质形成过程中釉质液的酸化。在啮齿动物中，分泌和成熟成釉细胞SLC 26 A4免疫阳性。染色是特别强的成熟成釉细胞面临形成釉质的顶端膜。RT-PCR证实了在釉质器官中存在Slc 26 a4的mRNA转录本。SLC 26 A4免疫染色也见于矿化结缔组织，包括成牙本质细胞、成骨细胞、骨细胞、破骨细胞、骨衬里细胞、成牙骨质细胞和牙骨质细胞。然而，Slc 26 a4-null突变小鼠没有明显的牙齿表型。SLC 26 A4在成熟成釉细胞的顶端质膜中的存在与作为pH调节剂的潜在功能一致。SLC 26 A4似乎对成釉细胞功能并不重要，并且可能由其他pH调节剂补偿。
Ameloblasts need to regulate pH during the formation of enamel crystals, a process that generates protons. Solute carrier family 26A member 4 (SLC26A4, or pendrin) is an anion exchanger for chloride, bicarbonate, iodine, and formate. It is expressed in apical membranes of ion-transporting epithelia in kidney, inner ear, and thyroid where it regulates luminal pH and fluid transport. We hypothesized that maturation ameloblasts express SLC26A4 to neutralize acidification of enamel fluid in forming enamel. In rodents, secretory and maturation ameloblasts were immunopositive for SLC26A4. Staining was particularly strong in apical membranes of maturation ameloblasts facing forming enamel. RT-PCR confirmed the presence of mRNA transcripts for Slc26a4 in enamel organs. SLC26A4 immunostaining was also found in mineralizing connective tissues, including odontoblasts, osteoblasts, osteocytes, osteoclasts, bone lining cells, cellular cementoblasts, and cementocytes. However, Slc26a4-null mutant mice had no overt dental phenotype. The presence of SLC26A4 in apical plasma membranes of maturation ameloblasts is consistent with a potential function as a pH regulator. SLC26A4 does not appear to be critical for ameloblast function and is probably compensated by other pH regulators.