Novel alternative splice variants of NFIX and their diverse mRNA expression patterns in dairy goat.
Novel alternative splice variants of NFIX and their diverse mRNA expression patterns in dairy goat.
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DOI：
10.1016/j.gene.2015.05.062
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发表时间：
2015-09
期刊：
影响因子：
3.5
通讯作者：
Xiaoyan Zhang;Yang Zhou;C. Pan;C. Lei;R. Dang;Hong Chen;X. Lan
中科院分区：
文献类型：
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作者：
Xiaoyan Zhang;Yang Zhou;C. Pan;C. Lei;R. Dang;Hong Chen;X. Lan
核因子I/X(NFIX)是NFI家族的一员，对肌肉和大脑发育有贡献。许多编码选择性剪接异构体的基因在生物过程中发挥着潜在的但不同的作用。到目前为止，NFIX基因的转录变异体及其在奶山羊中的表达谱从未被阐明。在此，我们鉴定并验证了NFIXa和NFIXb两个新的转录本在奶山羊中的表达。与正常转录本(NFIX)相比，NFIXa变异体缺失第一外显子和第九外显子，而NFIXb变异体缺失第一、第七和第九外显子，在第七外显子5‘端，NFIXa变异体比正常转录本多69个核苷酸，而在第七外显子5’端，NFIXb变异体比正常转录本长66个核苷酸。实时荧光定量聚合酶链式反应结果显示，这三个变异体的表达水平存在显著差异。正常的NFIX变异体在肺中大量表达，NFIXa变异体在胰腺中高表达，NFIXb变异体在肺和胰腺中大量表达。此外，NFIXa变异体在肝、脾、脂肪、肠和睾丸中的表达水平显著高于正常NFIX和NFIXb变异体(P<；分别为0.01或0.05)。NFIXa变异体在脑内的表达显著高于NFIXb变异体(P<；P<；0.01)。这些发现表明，NFIXa亚型是奶山羊某些组织中含量最丰富的亚型。本研究首次报道了山羊NFIX基因的选择性剪接变异体及其表达谱。这将有助于阐明NFIX基因在奶山羊中的功能。
Thenuclear factor I/X(NFIX) is a member of NFI family and contributes to muscle and brain development. Numerous genes coding for alternative splicing isoforms play potential but different roles in the biological process. To date, transcript variants ofNFIXgene and their expression profiles have never been elucidated in dairy goat. Herein, we identified and verified the expression of two novel transcripts (NFIXa and NFIXb) ofNFIXgene in dairy goat. Compared with the normal transcript (NFIX), the NFIXa variant lacked the first and ninth exons, while the NFIXb variant lacked the first, seventh and ninth exons; the NFIXa variant was 69 nt longer than the normal transcript in the 5′ end site of the seventh exon, while the NFIXb variant was 66 nt longer in the 5′ end site of the seventh exon. Quantitative real-time PCR results showed that the expression levels of the three variants were significantly different. The normal NFIX variant was abundantly expressed in the lungs, the NFIXa variant was highly expressed in the pancreas, and the NFIXb variant was abundantly expressed in both the lung and the pancreas. Additionally, the NFIXa variant showed a significantly higher expression level than those of the normal NFIX and the alternative NFIXb variants in the liver, spleen, adipose, intestine and the testis (P< 0.01 orP< 0.05), respectively. Expression of the NFIXa variant in the brain was significantly higher than that of the NFIXb variant (P< 0.01). These findings suggest that the NFIXa isoform is the most abundant isoform in certain tissues of the dairy goat. This study represents the first report on alternative splicing variants of the goatNFIXgene and their expression profiles. It should help elucidate the function ofNFIXgene in dairy goat.