Bacteria-Responsive Biomimetic Selenium Nanosystem for Multidrug-Resistant Bacterial Infection Detection and Inhibition
Bacteria-Responsive Biomimetic Selenium Nanosystem for Multidrug-Resistant Bacterial Infection Detection and Inhibition
复制标题
用于多重耐药细菌感染检测和抑制的细菌响应仿生硒纳米系统
DOI：
10.1021/acsnano.9b05766
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发表时间：
2019-12-01
期刊：
影响因子：
17.1
通讯作者：
Liu, Jie
中科院分区：
文献类型：
--
作者：
Lin, Ange;Liu, Yanan;Liu, Jie
耐多药细菌感染因其高致死率而对公众健康构成严重威胁。值得注意的是，抗生素的过早降解和不发达的成像能力仍然是一个挑战。本文提出了一种响应细菌感染微环境的硒纳米系统，作为一种抗生素替代品，通过联合策略检测和抑制耐甲氧西林金黄色葡萄球菌（MRSA）。利用天然红细胞膜（RBCM）和细菌反应性明胶纳米颗粒（GNPs），构建了Ru-Se@GNP-RBCM纳米体系，用于有效递送ru -配合物修饰的硒纳米颗粒（Ru-Se NPs）。利用天然RBCM，免疫系统清除率降低，外毒素被有效中和。GNPs可被明胶酶原位降解；因此，Ru-Se NPs被释放出来破坏细菌细胞。钌硒NPs具有较强的荧光成像能力，能准确监测感染治疗过程。此外，两种mrsa感染小鼠模型证实了良好的体内细菌清除和促进伤口愈合过程。综上所述，上述优点证明制备的纳米系统是一种有前途的抗生素替代品，可以对抗日益威胁的多重耐药细菌。
Multidrug-resistant (MDR) bacterial infections are a severe threat to public health owing to their high risk of fatality. Noticeably, the premature degradation and undeveloped imaging ability of antibiotics still remain challenging. Herein, a selenium nanosystem in response to a bacteria-infected microenvironment is proposed as an antibiotic substitute to detect and inhibit methicillin-resistant Staphylococcus aureus (MRSA) with a combined strategy. Using natural red blood cell membrane (RBCM) and bacteria-responsive gelatin nanoparticles (GNPs), the Ru-Se@GNP-RBCM nanosystem was constructed for effective delivery of Ru-complex-modified selenium nano particles (Ru-Se NPs). Taking advantage of natural RBCM, the immune system clearance was reduced and exotoxins were neutralized efficiently. GNPs could be degraded by gelatinase in pathogen-infected areas in situ; therefore, Ru-Se NPs were released to destroy the bacteria cells. Ru-Se NPs with intense fluorescence imaging capability could accurately monitor the infection treatment process. Moreover, excellent in vivo bacteria elimination and a facilitated wound healing process were confirmed by two kinds of MRSA-infected mice models. Overall, the above advantages proved that the prepared nanosystem is a promising antibiotic alternative to combat the ever-threatening multidrug-resistant bacteria.