Massive bone reconstruction with heat-treated bone graft loaded autologous bone marrow-derived strom al cells andβ-tricalcium phosphate composites in canine models
Massive bone reconstruction with heat-treated bone graft loaded autologous bone marrow-derived strom al cells andβ-tricalcium phosphate composites in canine models
复制标题
在犬模型中使用热处理骨移植负载自体骨髓源性基质细胞和β-磷酸三钙复合材料进行大规模骨重建
作者：
Koyanagi H;Ae K;Maehara H;Yuasa M;Masaoka T;Yamada T;Taniyama T;Saito M;Funauchi Y;Yoshii T;Okawa A;Sotome S
骨髓源性基质细胞（BMSCs）含有能够形成各种间充质组织的间充质干细胞。我们假设BMSCs和β-磷酸三钙（β-TCP）复合材料将促进大尺寸自体失活骨移植物的重塑;因此，本研究的目的是评价复合材料对自体失活骨移植物重塑的影响。将在含有地塞米松（10− 7 M）的培养基中培养的自体BMSC在低压下加载到多孔β-TCP颗粒中。将这些BMSC/TCP复合材料放入自体热处理骨的骨髓腔中（股骨骨干，65 mm长，100 ℃，30 min），并放回采集部位。在对侧，以与对侧相同的方式使用不含BMSC的β-TCP作为对照。与对照组相比，BMSC/TCP复合材料治疗导致移植物中心皮质骨的厚度、骨矿物质密度和成熟骨体积显著增加。组织学分析显示BMSC负载组中有成熟的再生骨。这些结果表明，BMSC/TCP复合材料促进了大尺寸失活骨移植部位的骨再生和成熟。这种方法有可能应用于临床重建大的骨缺损，如那些与骨肿瘤。© 2013 Orthopathy Research Society由Wiley Periodicals，Inc.出版。J Orthop Res 31：1308-1316，2013
Bone marrow‐derived stromal cells (BMSCs) contain mesenchymal stem cells that are capable of forming various mesenchymal tissues. We hypothesized that BMSCs and β‐tricalcium phosphate (β‐TCP) composites would promote the remodeling of large‐sized autologous devitalized bone grafts; therefore, the aim of this study was to evaluate the effects of the composites on the remodeling of autologous devitalized bone grafts. Autologous BMSCs cultured in culture medium containing dexamethasone (10−7M) were loaded into porous β‐TCP granules under low‐pressure. Theses BMSC/TCP composites were put into the bone marrow cavity of autologous heat‐treated bone (femoral diaphysis, 65‐mm long, 100°C, 30 min) and put back to the harvest site. In the contralateral side, β‐TCP without BMSC were used in the same manner as the opposite side as the control. Treatment with the BMSC/TCP composites resulted in a significant increase in thickness, bone mineral density, and matured bone volume of the cortical bone at the center of the graft compared to the control. Histological analysis showed matured regenerated bone in the BMSC loaded group. These results indicate that BMSC/TCP composites facilitated bone regeneration and maturation at the graft site of large‐sized devitalized bone. This method could potentially be applied for clinical use in the reconstruction of large bone defects such as those associated with bone tumors. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1308–1316, 2013