Characterization of vaginal microbiota in Chinese women with cervical squamous intra-epithelial neoplasia
Characterization of vaginal microbiota in Chinese women with cervical squamous intra-epithelial neoplasia
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中国宫颈鳞状上皮内瘤变女性阴道微生物群特征
DOI：
10.1136/ijgc-2020-001341
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发表时间：
2020-10-01
影响因子：
4.8
通讯作者：
Ying, Chunmei
中科院分区：
文献类型：
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作者：
Wu, Mengying;Gao, Jing;Ying, Chunmei
目的虽然持续性人乳头瘤病毒（HPV）感染是宫颈鳞状上皮内瘤变的主要原因，但阴道微生物群与不同级别鳞状上皮内瘤变的关系尚不明确。我们探讨了阴道微生物群与宫颈鳞状上皮内瘤变进展之间的可能关系。方法对复旦大学附属妇产科医院妇产科69例产妇进行临床调查。使用Illumina MiSeq对条形码16 S rRNA基因片段（V3-4）进行深度测序，对三组女性的阴道细菌组成进行表征。排除标准为任何既往子宫切除术、宫颈癌或其他下生殖器癌病史和/或宫颈破坏性治疗。患有自身免疫性疾病、艾滋病毒阳性、在采样前15天内接受抗生素治疗或在采样前48小时内进行性交或冲洗的妇女也被排除在外。使用单向ANOVA计算年龄和微生物比例的P值，使用卡方检验计算HPV状态和群落状态类型（CST）的P值。结果31例无上皮内病变或恶性肿瘤、22例有低度鳞状上皮内病变（LSIL）和16例有高度鳞状上皮内病变（HSIL）的妇女阴道菌群组成以乳杆菌为主，普雷沃菌和链球菌在HSIL组中增多。宫颈疾病进展与高危型HPV感染的患病率相关。鳞状上皮内瘤变使阴道细菌群落结构从CSTs IV型转变为II型。微生物群多样性在CST II型和IV型中更为明显（p
Objectives Although persistent human papillomavirus (HPV) infection is a major cause of cervical squamous intra-epithelial neoplasia, the relationship between vaginal microbiota and different grades of squamous intra-epithelial neoplasia is not well established. We explored the possible relationship between the vaginal microbiota and the progression of cervical squamous intra-epithelial neoplasia. Methods We evaluated 69 women who attended the Obstetrics and Gynecology Hospital of Fudan University. The vaginal bacterial composition of three groups of women was characterized by deep sequencing of bar-coded 16S rRNA gene fragments (V3-4) using Illumina MiSeq. Exclusion criteria were any previous hysterectomy, history of cervical or other lower genital cancer, and/or destructive therapy of the cervix. Women who had autoimmune disorders, who were HIV positive, who received antibiotics within 15 days of sampling, or who had engaged in sexual intercourse or douching within 48 hours prior to sampling were also excluded. P values for age and proportions of organisms were calculated using one-way ANOVA and p values for HPV status and community state types (CSTs) were calculated using a chi(2)test. Results The vaginal bacterial composition of three groups of women, those without an intra-epithelial lesion or malignancy (n=31), those with a low-grade squamous intra-epithelial lesion (LSIL) (n=22), and those with a high-grade squamous intra-epithelial lesion (HSIL) (n=16) were analyzed.Lactobacilluswas the most dominant genus overall.PrevotellaandStreptococcuswere increased in the HSIL group. Cervical disease progression was associated with the prevalence of high-risk HPV infection. Squamous intra-epithelial neoplasia converted the vaginal bacterial community structure from CSTs IV to II. Microbiota diversity was more pronounced in CST types II and IV (p