Brassica napus PHR1 gene encoding a MYB-like protein functions in response to phosphate starvation.
Brassica napus PHR1 gene encoding a MYB-like protein functions in response to phosphate starvation.
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DOI：
10.1371/journal.pone.0044005
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Li XB
中科院分区：
文献类型：
--
作者：
Ren F;Guo QQ;Chang LL;Chen L;Zhao CZ;Zhong H;Li XB
磷是植物发育所必需的营养元素之一。本研究从甘蓝型油菜中分离到了编码MYB转录激活子的BnPHR1。核定位和转录激活能力的表征表明BnPHR1是一个转录激活因子。组织表达和组织化学分析表明，缺磷条件下，BnPHR1主要在根中表达，并受到外源Pi在根中转录水平的调节。此外，BnPHR1在拟南芥和甘蓝型油菜中的过表达显著增强了Pi饥饿诱导基因的表达，包括编码高亲和力Pi转运蛋白的ATPT2和BnPT2。此外，在拟南芥和甘蓝型油菜中，BnPHR1可以在体内结合ATPT2和BnPT2的启动子序列。可能是由于ATPT2和BnPT2，甚至更多的高亲和力Pi转运体的激活，过量的Pi在转基因植物中积累，导致对细胞至关重要的Pi毒性，从而延缓了植物的生长。综上所述，BnPHR1作为关键的调控因子，受外源Pi的调控，直接激活这些基因，促进植物生长对Pi的吸收和稳态。
Phosphorus (P) is one of the essential nutrient elements for plant development. In this work, BnPHR1 encoding a MYB transcription activator was isolated from Brassica napus. The characterization of nuclear localization and transcription activation ability suggest BnPHR1 is a transcriptional activator. The tissue expression and histochemical assay showed that BnPHR1 was predominantly expressed in roots and modulated by exogenous Pi in transcriptional level in roots under Pi deficiency conditions. Furthermore, overexpression of BnPHR1 in both Arabidopsis and B. napus remarkably enhanced the expression of the Pi-starvation-induced genes including ATPT2 and BnPT2 encoding the high-affinity Pi transporter. Additionally, BnPHR1 can in vivo bind the promoter sequence of ATPT2 and BnPT2 in both Arabidopsis and B. napus. Possibly, due to the activation of ATPT2 and BnPT2, or even more high-affinity Pi transporters, the excessive Pi was accumulated in transgenic plants, resulting in the crucially Pi toxicity to cells and subsequently retarding plant growth. Given the data together, BnPHR1, as crucial regulator, is regulated by exogenous Pi and directly activates those genes, which promote the uptake and homeostasis of Pi for plant growth.
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