Binding rather than metabolism may explain the interaction of two food-grade Lactobacillus strains with zearalenone and its derivative α-zearalenol
Binding rather than metabolism may explain the interaction of two food-grade Lactobacillus strains with zearalenone and its derivative α-zearalenol
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DOI：
10.1128/aem.68.7.3545-3549.2002
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发表时间：
2002-07-01
影响因子：
4.4
通讯作者：
Mykkänen, H
中科院分区：
文献类型：
--
作者：
El-Nezami, H;Polychronaki, N;Mykkänen, H
研究了镰刀菌毒素玉米赤霉烯酮（ZEN）及其衍生物α-玉米赤霉烯醇（α-ZOL）与两株食品级乳杆菌的相互作用。将真菌毒素（2 μ g ml（-1））与鼠李糖乳杆菌菌株GG或L.鼠李糖菌株LC 705。从细菌沉淀中回收了相当大比例（38 - 46%）的两种毒素，在培养基上清液和沉淀甲醇提取物的高效液相色谱图中未检测到ZEN和α-ZOL的降解产物。热处理和酸处理的细菌都能够去除毒素，这表明结合而不是代谢是毒素从培养基中去除的机制。ZEN或α-ZOL与冻干L. rhamnosus GG和L. rhamnosus LC 705的反应迅速：约55%的毒素在与细菌混合后立即结合。结合依赖于细菌浓度，ZEN与α-ZOL的共孵育显著影响毒素结合的百分比，表明这些毒素可能在细菌表面上共享相同的结合位点。这些结果可用于开发一种新的方法，从食品和饲料中去除霉菌毒素。
The interaction between two Fusarium mycotoxins, zearalenone (ZEN) and its derivative alpha-zearalenol (alpha-ZOL), with two food-grade strains of Lactobacillus was investigated. The mycotoxins (2 mug ml(-1)) were incubated with either Lactobacillus rhamnosus strain GG or L. rhamnosus strain LC705. A considerable proportion (38 to 46%) of both toxins was recovered from the bacterial pellet, and no degradation products of ZEN and alpha-ZOL were detected in the high-performance liquid chromatograms of the supernatant of the culturing media and the methanol extract of the pellet. Both heat-treated and acid-treated bacteria were capable of removing the toxins, indicating that binding, not metabolism, is the mechanism by which the toxins are removed from the media. Binding of ZEN or alpha-ZOL by lyophilized L. rhamnosus GG and L. rhamnosus LC705 was a rapid reaction: approximately 55% of the toxins were bound instantly after mixing with the bacteria. Binding was dependent on the bacterial concentration, and coincubation of ZEN with alpha-ZOL significantly affected the percentage of the toxin bound, indicating that these toxins may share the same binding site on the bacterial surface. These results can be exploited in developing a new approach for detoxification of mycotoxins from foods and feeds.