Effects of L-ascorbic acid 2-phosphate magnesium salt on the properties of human gingival fibroblasts
Effects of L-ascorbic acid 2-phosphate magnesium salt on the properties of human gingival fibroblasts
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DOI：
10.1111/j.1600-0765.2011.01430.x
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发表时间：
2012-04-01
影响因子：
3.5
通讯作者：
Murakami, S.
中科院分区：
文献类型：
--
作者：
Tsutsumi, K.;Fujikawa, H.;Murakami, S.
背景和目的：L-抗坏血酸2-磷酸镁盐（APM）是一种L-抗坏血酸（阿萨）衍生物，具有提高阿萨稳定性和生物活性的作用。本研究旨在探讨APM与阿萨对人牙龈成纤维细胞功能和特性的影响。材料和方法：人牙龈成纤维细胞在APM或L-抗坏血酸钠（AsANa）存在或不存在的情况下孵育。用高效液相色谱法测定细胞内阿萨含量。通过ELISA和实时RT-PCR测量胶原合成。用荧光试剂定量过氧化氢（H2 O2）诱导的细胞内活性氧（ROS），用钙黄绿素乙酰氧基甲酯评估细胞损伤。结果：APM能显著持续地促进细胞内阿萨的合成，并促进1型胶原的合成和mRNA的表达。此外，APM通过抑制H2 O2诱导的细胞内ROS减少细胞损伤，并通过抑制TNF-α诱导的细胞内ROS抑制白细胞介素-8的产生。结论：APM对牙龈成纤维细胞的摄取、胶原合成、细胞损伤及IL-8表达的抑制作用均上级AsANa。提示局部应用APM有助于预防牙周病。
Background and Objective: L-Ascorbic acid 2-phosphate magnesium salt (APM) is an L-ascorbic acid (AsA) derivative developed to improve AsA stability and display effective biochemical characteristics. This study aimed to investigate the effects of APM on the functions and properties of human gingival fibroblasts with respect to the prevention of periodontal disease in comparison with those of AsA.Material and Methods: Human gingival fibroblasts were incubated in the presence or absence of APM or L-ascorbic acid sodium salt (AsANa). Intracellular AsA was analysed by HPLC. Collagen synthesis was measured by ELISA and real-time RT-PCR. Intracellular reactive oxygen species (ROS) induced by hydrogen peroxide (H2O2) were quantified using a fluorescence reagent, and cell damage was estimated with calcein acetoxymethyl ester. Furthermore, intracellular ROS induced by tumor necrosis factor-alpha (TNF-alpha) were quantified, and expression of TNF-alpha-induced interleukin-8 expression, which increases due to inflammatory reactions, was measured by ELISA and real-time RT-PCR.Results: APM remarkably and continuously enhanced intracellular AsA and promoted type 1 collagen synthesis and mRNA expression. Furthermore, APM decreased cell damage through the suppression of H2O2-induced intracellular ROS and inhibited interleukin-8 production through the suppression of TNF-alpha-induced intracellular ROS. These effects of APM were superior to those of AsANa.Conclusion: These results suggest that APM is more effective than AsANa in terms of intake, collagen synthesis, decreasing cell damage and inhibiting interleukin-8 expression in human gingival fibroblasts. This suggests that local application of APM can help to prevent periodontal disease.