Oxidative stress and metabolism in animal model of colitis induced by dextran sulfate sodium
Oxidative stress and metabolism in animal model of colitis induced by dextran sulfate sodium
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DOI：
10.1111/j.1440-1746.2007.04890.x
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发表时间：
2007-11-01
影响因子：
4.1
通讯作者：
Streck, Emilio L.
中科院分区：
文献类型：
--
作者：
Damiani, Carlos R.;Benetton, Cesar A. F.;Streck, Emilio L.
背景和目的：溃疡性结肠炎是一种慢性胃肠道炎症性疾病。其病因尚不清楚，但似乎是由于免疫反应失调，吞噬性白细胞浸润到粘膜粘膜上皮。免疫细胞产生和释放活性氧在结肠炎的病理生理学中起着至关重要的作用。本研究旨在探讨N-乙酰半胱氨酸（NAC）和去铁胺（DFX）对葡聚糖硫酸钠（DSS）诱导的大鼠结肠炎的治疗作用。方法：通过测定大鼠肠道氧化应激指标、线粒体功能、炎症反应和肠道组织病理学改变，观察NAC和DFX对DSS诱导的大鼠结肠炎的治疗作用。DSS增加了白色血细胞计数，NAC和DFX不能阻止这种作用。然而，DSS增加大鼠结肠线粒体呼吸链复合物IV，NAC和DFX阻止了这种改变。此外，DSS处理大鼠结肠中硫代巴比妥酸反应物质增加。NAC和DFX一起使用时，可以防止这种影响。复合物II和琥珀酸脱氢酶不受DSS的影响，作为蛋白质羰基container.Conclusions：据推测，NAC和DFX可能是有用的治疗结肠炎，但需要进一步的研究来澄清这些影响。
Background and Aim: Ulcerative colitis is a chronic inflammatory disease of the gastrointestinal tract. Its etiology remains unclear, but it appears to result from a dysregulated immune response, with infiltration of phagocytic leukocytes into the mucosal interstitium. The production and release of reactive oxygen species by immune cells seems to play a crucial role in physiopathology of colitis. The aim of this work was to evaluate the effects of N-acetylcysteine (NAC) and deferoxamine (DFX) in the treatment of colitis induced by dextran sulfate sodium (DSS).Methods: The effects of NAC and DRX on rats with DSS-induced colitis were determined by measuring intestinal parameters of oxidative stress and mitochondrial function, inflammatory response and bowel histopathological alterations.Results: DSS increased white blood cells count and NAC and DFX did not prevent this effect. However, DSS increased mitochondrial respiratory chain complex IV in colon of rats and NAC and DFX prevented this alteration. In addition, thiobarbituric acid reactive substances were increased in colon of DSS-treated rats. NAC and DFX, when taken together, prevented this effect. Complex II and succinate dehydrogenase were not affected by DSS, as protein carbonyl content.Conclusions: It is speculated that NAC and DFX might be useful for treatment of colitis, but further research is necessary to clarify these effects.