Expressão heteróloga da EMA-2 (equi merozoite antigen) de Theileria equi em Pichia pastoris com potencial utilização em imunobiológicos
Expressão heteróloga da EMA-2 (equi merozoite antigen) de Theileria equi em Pichia pastoris com potencial utilização em imunobiológicos
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毕赤酵母和马泰勒菌表达 EMA-2（马裂殖子抗原）异源，利用免疫生物学的潜力
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发表时间：
2014
期刊：
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通讯作者：
F.E.P Leite
中科院分区：
文献类型：
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作者：
Ana Muñoz Vianna;Relber Aguiar Gonçales;Ana Paula de Souza Stori de Lara;L. D. S. Pinto;Leandro Quintana Nizoli;F.E.P Leite
马泰勒虫是马梨形虫病的病原体，在巴西和许多其他热带和亚热带国家流行。它被认为是马最重要的疾病之一，会导致动物健康问题和重大经济损失。马裂殖子抗原-2（EMA-2）是寄生虫的主要表面蛋白，在寄生虫不同的周期阶段表达，并诱导感染动物产生免疫应答，是诊断的可能候选抗原。将EMA-2基因克隆到毕赤酵母中进行表达，并对重组蛋白EMA-2（rEMA-2）进行Western Blot和间接ELISA鉴定。与标准ELISA相比，rEMA-2的特异性为90.9%，敏感性为83.3%，特异性为80.6%，敏感性为75.0%，优于免疫荧光法（上级），提示rEMA-2在泰勒虫病的免疫诊断中具有良好的应用前景
Theileria equi, the causative agent of equine piroplasmosis, is endemic in Brazil and many other tropical and subtropical countries. It is considered one of the most important diseases of horses causing animal health problems and significant economic loss. The Protein equi merozoite antigen-2 (EMA-2) is a major surface protein that is expressed in different parasite cycle stages and induces immune response in infected animals, being a possible candidate to be used in diagnose. EMA-2 gene was cloned and expressed in the yeast Pichia pastoris, and the recombinant protein EMA-2 (rEMA-2) was characterized by Western Blot and indirect ELISA using equine positive sera. The ELISA results demonstrated a specificity of 90.9% and a sensitivity of 83.3% compared to the standard ELISA and being superior to immunofluorescence (80.6% of specificity and 75.0% of sensitivity) suggesting that the rEMA-2 expressed in P. pastoris is a promising antigen to be used as a tool in immunodiagnostic of theileriasis