Pathogen-inducible OsMPKK10.2-OsMPK6 cascade phosphorylates the Raf-like kinase OsEDR1 and inhibits its scaffold function to promote rice disease resistance
Pathogen-inducible OsMPKK10.2-OsMPK6 cascade phosphorylates the Raf-like kinase OsEDR1 and inhibits its scaffold function to promote rice disease resistance
复制标题
病原体诱导的 OsMPKK10.2-OsMPK6 级联磷酸化 Raf 样激酶 OsEDR1 并抑制其支架功能，促进水稻抗病性
DOI：
10.1016/j.molp.2021.01.008
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发表时间：
2021-04-05
期刊：
影响因子：
27.5
通讯作者：
Wang, Shiping
中科院分区：
文献类型：
--
作者：
Ma, Haigang;Li, Juan;Wang, Shiping
有丝分裂原活化蛋白激酶（MAPK）级联调节无数的植物生物学过程，包括抗病性。植物基因组编码大量的MAPK激酶激酶（MAPKKKs），其可分为两个亚家族，即MEKK-like激酶和Raf-like激酶。到目前为止，MEKK样MAPKKKs的功能已经得到了比较好的表征，但Raf-like MAPKKKs在植物MAPK级联中的作用仍然不太清楚。在这里，我们报告的作用OsEDR 1，Raf-like MAPKKK，在水稻响应细菌病原体黄单胞菌的MAPK级联的调节。oryzicola（Xoc）.我们发现OsEDR 1通过物理相互作用抑制OsMPKK10.2（MAPK激酶）活性。在Xoc感染后，OsMPKK10.2在S304处被磷酸化以激活OsMPK 6（MAPK）。有趣的是，激活的OsMPK 6在S861处磷酸化OsEDRl，这使OsEDRl不稳定，从而释放OsMPKK10.2的抑制，导致OsMPKK10.2活性增加，从而增强水稻对Xoc的抗性。总之，这些结果提供了新的见解Raf-like激酶在植物免疫的MAPK级联调节的功能。
Mitogen-activated protein kinase (MAPK) cascades regulate a myriad of plant biological processes, including disease resistance. Plant genomes encode a large number of MAPK kinase kinases (MAPKKKs) that can be divided into two subfamilies, namely MEKK-like kinases and Raf-like kinases. Thus far, the functions of MEKK-like MAPKKKs have been relatively well characterized, but the roles of Raf-like MAPKKKs in plant MAPK cascades remain less understood. Here, we report the role of OsEDR1, a Raf-like MAPKKK, in the regulation of the MAPK cascade in rice response to the bacterial pathogen Xanthomonas oryzae pv. oryzicola (Xoc). We found that OsEDR1 inhibits OsMPKK10.2 (a MAPK kinase) activity through physical interaction. Upon Xoc infection, OsMPKK10.2 is phosphorylated at S304 to activate OsMPK6 (a MAPK). Interestingly, activated OsMPK6 phosphorylates OsEDR1 at S861, which destabilizes OsEDR1 and thus releases the inhibition of OsMPKK10.2, leading to increased OsMPKK10.2 activity and enhanced resistance of rice plants to Xoc. Taken together, these results provide new insights into the functions of Raf-like kinases in the regulation of the MAPK cascade in plant immunity.