CHARACTERIZATION OF ANAEROVIBRIO-BURKINABENSIS SP-NOV, A LACTATE-FERMENTING BACTERIUM ISOLATED FROM RICE FIELD SOILS
CHARACTERIZATION OF ANAEROVIBRIO-BURKINABENSIS SP-NOV, A LACTATE-FERMENTING BACTERIUM ISOLATED FROM RICE FIELD SOILS
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DOI：
10.1099/00207713-42-3-390
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发表时间：
1992-07-01
期刊：
影响因子：
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通讯作者：
GARCIA, JL
中科院分区：
文献类型：
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作者：
OUATTARA, AS;TRAORE, AS;GARCIA, JL
以乳酸为唯一碳源和能源，从稻田土壤中分离到一株严格厌氧的革兰氏阴性细菌。 这些细胞是不形成孢子的、能动的、弯曲的杆状细胞。 最佳生长发生在35 ℃和pH 6.8。 未观察到NaCl要求。 生长需要维生素。 我们的分离物，菌株B4 B 0 T（T =型菌株），发酵的丙酮酸、富马酸、苹果酸、柠檬酸、二羟基丙酮、果糖、1，2-丙二醇、谷氨酸和天冬氨酸转化为乙酸、丙酸、琥珀酸和痕量氢。 菌株B4 B 0 T不使用核糖或甘油作为能源，尽管甘油降解主要产生1，3-丙二醇。 三价铁被任意还原。 硝酸盐和硫酸盐没有减少。 存在细胞色素B。 DNA的鸟嘌呤加胞嘧啶含量为44.1 +/- 0.1 mol%。 我们认为菌株B4 B 0（= DSM 6283）应该是Anaerovibrio burkinabensis的一个新种的模式菌株。
A strictly anaerobic, gram-negative bacterium was isolated from rice field soils by using lactate as a sole carbon and energy source. The cells were non-spore-forming, motile, curved rods. Optimal growth occurred at 35-degrees-C and pH 6.8. No NaCl requirement was observed. Vitamins were required for growth. Our isolate, strain B4B0T (T = type strain), fermented pyruvate, fumarate, malate, citrate, dihydroxyacetone, fructose, 1,2-propanediol, glutamate, and aspartate to acetate, propionate, succinate, and traces of hydrogen. Strain B4B0T did not use ribose or glycerol as an energy source, although glycerol degradation produced mainly 1,3-propanediol. Ferric iron was facultatively reduced. Nitrate and sulfate were not reduced. Cytochrome b was present. The guanine-plus-cytosine content of the DNA was 44.1 +/- 0.1 mol%. We propose that strain B4B0 (= DSM 6283) should be the type strain of a new species in the genus Anaerovibrio, Anaerovibrio burkinabensis.