Novel families of antimicrobial peptides with multiple functions from skin of Xizang plateau frog, Nanorana parkeri
Novel families of antimicrobial peptides with multiple functions from skin of Xizang plateau frog, Nanorana parkeri
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西藏高原蛙皮肤的多功能抗菌肽新家族
DOI：
10.1016/j.biochi.2010.01.025
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发表时间：
2010-05-01
期刊：
影响因子：
3.9
通讯作者：
Yu, Haining
中科院分区：
文献类型：
--
作者：
Lu, Zekuan;Zhai, Lei;Yu, Haining
西藏高原蛙（Nanorana parkeri）是在中国西藏拉萨捕获的，仅生活在亚热带高原，那里有强烈的紫外线辐射和长时间的日照。针对其恶劣的生存环境，研究了其对微生物和环境胁迫的天然防御能力。本研究从嗜热厌氧菌皮肤分泌物中分离纯化了三种抗菌肽（AMPS）。parkeri。同时，还从日本血吸虫的皮肤cDNA文库中克隆了编码该基因的cDNA序列。parkeri。经结构鉴定，其中两个肽段属于Japonicin-1家族，分别命名为Japonicin-1 Npa（FLLFPLMCKIQGKC）和Japonicin-1 Npb（FVLPLVMCKILRKC）。第三个肽分离命名为Parkerin与一个独特的序列GWANTLKNVAGGLCKITGAA没有显示出任何已知的两栖动物AMP的相似性。对三种AMPS的多功能性（抗氧化、MCD、溶血等）进行了检测。用圆二色谱（CD）测定了它们的溶液结构，用扫描电镜（SEM）研究了它们的抗菌机理，结果与它们的功能特性非常一致。目前的结果表明，这些新的多功能AMP可能在防御N。parkeri对抗环境氧化应激和病原微生物的能力，这可能部分揭示了这些生活在高原的两栖动物的生态适应。(C)2010年Elsevier Masson SAS。All rights reserved.
Xizang plateau frog (Nanorana parkeri) captured in Lhasa, Tibet, China, solely lives in the subtropical plateau, where there is strong ultraviolet radiation and long duration of sunshine. Considering its harsh living environment, the frog's innate defense against microbes and environmental stress was investigated. In current study, three antimicrobial peptides (AMPS) were purified and characterized from the skin secretion of N. parkeri. The coding cDNA sequences were also cloned from the skin cDNA library of N. parkeri. By structural characterization, two peptides were identified belonging to Japonicin-1 family, and named as Japonicin-1Npa (FLLFPLMCKIQGKC) and Japonicin-1 Npb (FVLPLVMCKILRKC). The third peptide isolated named Parkerin with a unique sequence of GWANTLKNVAGGLCKITGAA did not show similarity to any known amphibian AMPs. Multi-functions of three AMPS were examined (antioxidant, MCD, hemolytic etc). Their solution structures determined by CD and antimicrobial mechanisms investigated by SEM are very well consistent with their functional characters. Current result suggests that these novel multi-functional AMPs could play an important role in defending N. parkeri against environmental oxidative stress and pathogenic microorganisms, which may partially reveal the ecological adaptation of these plateau-living amphibians. (C) 2010 Elsevier Masson SAS. All rights reserved.