EGF content in the gastrointestinal tract of rats: effect of age and fasting/feeding.
EGF content in the gastrointestinal tract of rats: effect of age and fasting/feeding.
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大鼠胃肠道中的 EGF 含量：年龄和禁食/进食的影响。
DOI：
10.1152/ajpgi.1989.256.5.g856
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Koldovsky,O
中科院分区：
文献类型：
--
作者：
Schaudies,RP;Grimes,J;Davis,D;Rao,RK;Koldovsky,O
免疫反应性大鼠表皮生长因子（EGF）测定胰腺和粘膜和胃，十二指肠，空肠，空肠中段，回肠，和结肠的喂养或禁食5和12天龄的哺乳动物，3至4个月大的成年雄性大鼠使用同源放射免疫法。哺乳动物胰腺中的EGF水平低于成年人，并且不受禁食的影响。乳鼠胃肠道粘膜和肠腔EGF水平均高于成年鼠。禁食导致乳鼠胃肠道EGF水平显著下降，但成年大鼠的变化极小。我们的研究结果表明，胃肠道中EGF的含量依赖于年龄和饮食状况。牛奶中含有大量的EGF（EGF）。Koldovsky和W. Thornburg，J. Pediatr.肠胃Nutr. 6：172-196，1987），并且标记的EGF在相当大程度上被乳鼠的胃肠道吸收（P.A. Gonella等人，J. Clin. Invest. 80：22-32，1987：W. Thornburg等人，Am. J.Physiol.246：G80-G85，1984），我们目前的研究表明，牛奶是哺乳期EGF的重要来源。
Immunoreactive rat epidermal growth factor (EGF) was measured in the pancreas and in the mucosa and lumen of the stomach, duodenum, jejunum, midjejunum, ileum, and colon of fed or fasted 5- and 12-day-old suckling, and 3- to 4-month-old adult male rats using a homologous radioimmunoassay. The EGF levels in the pancreas in sucklings were lower than in adults and were unaffected by fasting. Both gastrointestinal mucosal and luminal EGF levels were higher in suckling rats than in adults. Fasting caused a significant decrease in gastrointestinal levels of EGF in the suckling rats but resulted in minimal changes in the adults. Our results show that the content of EGF in gastrointestinal tract is dependent on both age and dietary status. Together with the fact that milk contains a large amount of EGF (O. Koldovsky and W. Thornburg, J. Pediatr. Gastro. Nutr. 6: 172-196, 1987) and that labeled EGF is absorbed to a considerable extent by the gastrointestinal tract of suckling rats (P.A. Gonella et al., J. Clin. Invest. 80: 22-32, 1987: W. Thornburg et al., Am. J. Physiol. 246: G80-G85, 1984), our present study implicates milk as an important source of EGF in the suckling period.