Identification and functional analysis of Hyphantria cunea nucleopolyhedrovirus iap genes
Identification and functional analysis of Hyphantria cunea nucleopolyhedrovirus iap genes
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DOI：
10.1016/j.virol.2004.01.002
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发表时间：
2004-04-10
期刊：
影响因子：
3.7
通讯作者：
Kobayashi, M
中科院分区：
文献类型：
--
作者：
Ikeda, M;Yanagimoto, K;Kobayashi, M
美国白蛾核型多角体病毒(HycuNPV)感染SPIM细胞后，最早在感染后4h即可保护细胞免受放线菌素D(ActD)诱导的细胞凋亡。Southern杂交分析表明，HycuNPV基因组中含有三个凋亡抑制基因，分别命名为hycu-iap1、hycu-iap2和hycu-iap3，因为它们的氨基酸序列与其他杆状病毒中的iap1同源。瞬时表达分析表明，Hycu-IAP3阻断了放线菌素D诱导的细胞凋亡，并挽救了p35缺陷突变株AcMNPV的复制，而Hycu-IAP1和Hycu-IAP2没有任何抗凋亡作用。RNA干扰抑制Hycu-iap3在SPIM细胞中的表达诱导细胞凋亡这些结果表明，Hycu-IAP3在HycuNPV感染允许的SPIM细胞过程中对细胞凋亡的阻断是必不可少的。(C)2004 Elsevier Inc.保留所有权利。
Hyphantria cunea nucleopolyhedrovirus (HycuNPV) infection protected SpIm cells from actinomycin D (ActD)-induced apoptosis as early as 4 h postinfection. Analysis by Southern hybridization revealed that the HycuNPV genome possessed three members of inhibitor of apoptosis genes (iaps) that were designated as hycu-iap1, hycu-iap2, and hycu-iap3 because of their amino acid sequence homology with iaps identified in other baculoviruses. Functional analysis of Hycu-IAPs by transient expression assay in Sf9 cells revealed that Hycu-IAP3 blocked apoptosis induced by actinomycin D and rescued replication of p35 deficient-mutant AcMNPV, while Hycu-IAP1 and Hycu-IAP2 did not show any anti-apoptotic functions. Knockdown of hycu-iap3 expression by RNAi during HycuNPV infection in SpIm cells induced apoptosis. These results indicate that Hycu-IAP3 is essential for blockage of apoptosis during HycuNPV infection of permissive SpIm cells. (C) 2004 Elsevier Inc. All rights reserved.